INSTITUTUL NAÅ¢IONAL DE CERCETARE-DEZVOLTARE - ICECHIM

3. Chemistry in medical and pharmaceutical applications - ONEW POLYSACCHARIDES WITH ALKALINE PHOSPHATASEMODULATORS EFFECTMădălina STĂICULESCU, Ioana NICU, Cristina STURZOIU, GheorgheSTOIANDepartment of Biochemistry and Molecular Biology, Faculty of Biology, University ofBucharest, 91-95, Splaiul Independentei, sect. 5, Bucharest, ROMANIAAlkaline phosphatases (EC 3.1.3.1), a group of dimeric enzymes 1 , have an importantrole in intestinal homeostasis and exists in three major forms: intestinal, placental and tissuenon-specific. These enzymes are involved in phosphomonoesters hydrolysis, in breakdown ofdietary cholesterol and also in calcium absorption 2 . Phosphatase expression and activity canbe modulated by some nutrition compounds, such as carbohydrates 3 . Literature data showedthat some polysaccharides have demonstrated prebiotic effect in vitro and in vivo 4 . The aimof our researches was to evaluate two new polysaccharides modulating agents for phosphataseactivity in Cobb’s 500 hybrid broilers and Wistar rats’ small intestines. In this context weused a β (2-1) polysaccharide (inulin) and a β (2-6) microbial polysaccharide (levan), the lastone produced by a nonpathogenic Gram negative bacterium, Z. mobilis. We analyzed thisparticular enzyme from duodenum, jejune and ileum after inulin or bacterial levan (2 mg/mL)treatment.Our results showed a significantly enzymatic activation effect on intestine Wistar rats,especially on ileum segment by both polysaccharides, more than 170% and 70%, respectivelycomparative with control. On the other hand, alkaline phosphatase from broilers small intestine wasactivated in duodenum and jejune segment only by inulin; ileum alkaline phosphates were 50%activated by microbial levan. Such modulation comportment can be explained by the interactionbetween polysaccharides and enzymes and its effect therefore enhance enzyme activity.1. McComb R. B., Bowers G. N., Posen S., Alkaline phosphatase, Plenum Press, New York, N.Y. 1979.2. Moog, F., Glazier, H.S. Comp Biochem Physiol, 42, 321-336, 1972.3. Lalles J.P. Nutr Rev, 68, 323-332, 2010.4. Kolida, S., Tuohy, K.., Gibson G. R., Br. J. Nutr. 87, S193 - S197, 2007.