1 Bioresources and biomaterials PTHE USE OF AMMONIUM PERSULFATE AS A NOVEL OXIDANT IN<strong>DE</strong>LIGNIFICATION TREATMENT OF DIFFERENT RAW MATERIALSManea Vasilica 1 * , Lăpugean Alexandru 2 , Necula Mădălina 2 ,Câmpeanu Gheorghe 3 , Israel-Roming Florentina 3 , Nicu Ioana 2 , Stoian Gheorghe 21 National Institute for Chemical - Pharmaceutical Research and Development - ICCF Bucharest,Microbial Biotechnology Department, * corresponding author: z18silvia@yahoo.com2 Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest3 Applied Biochemistry and Biotechnology Center BIOTECHNOL Bd. Mărăşti, no. 59Wastes and by-products from agro-industrial processes (non-grain and non-foodsources) are abundantly produced in the world every day, and have modest, if any,applications. In this circumstance, the second generation of biofuels production is thought touse lignocellulose feedstock such as raw material [1] . These wastes, sources of lignocelluloseconstitute important substrates in fermentative processes directed to biofuel production. Thestructural carbohydrates in the plant cell wall are wrapped up in lignin. Therefore, a veryeffective way, but not the only option to significantly increase biomass digestibility is lignindegradation or separation (delignification). The operation is aimed to increase the digestibilityof constituent sugars through increment in gross material pore size [2, 3] . Many ofdelignification methods employ mineral acid, alkaline or/and oxidative reaction conditionsthat lead to reduction of molecular weight of lignin and consequently to pass it into a solution[1-5] . The aim of this study was to evaluate the feasibility of delignification of differentlignocellulose feedstock (grape stalk, depleted stalk sweet sorghum, and wheat straw) usingammonium persulfate as oxidative agent. Treating finely divided lignocellulose material for 2h at 121 o C with diluted solution of ammonium persulfate (1% and 3% respectively) andextracting it with diluted alkali (1%) readily dilignifies all studied materials.References[1]. Naik S.N., Goud V.V., Rout P.K, Dalai A.K., 2010, Production of first and second generationbiofuels: A comprehensive review. Renewable and Sustainable Energy Reviews. 14 (February 2010), 578597 ,0136-4032[2]. Sierra R., Smith A., Granda C.B, Holtzapple M., 2008, Producing Fuels and Chemicals fromLignocellulosic Biomass. Chemical Engineering Progress. 104 8 (August 2008), S10S18 .[3]. Sánchez O., Sierra R., Alméciga-Díaz C.J., 2011, Delignification Process of Agro-Industrial Wastesan Alternative to Obtain Fermentable Carbohydrates for Producing Fuel. In Alternative Fuel, Manzanera, M. Ed.InTech, 2011, http://www.intechopen.com/books/alternative-fuel/delignification-process-of-agro-industrialwastes-an-alternative-to-obtain-fermentable-carbohydrates.[4]. Li B.Z., Balan V., Yuan Y.J., Dale B.E., 2010, Process optimization to convert forage and sweetsorghum bagasse to ethanol based on ammonia fiber expansion (AFEX) pretreatment. Bioresource Technology.101 4 (February 2010), 12851292 , 0960-8524[5]. Simmons B.A., Loque D., Blanch H.W., 2008, Next-generation biomass feedstocks for biofuelproduction. Genome Biology. 9 242 (December 2008), 1465-6914
1. 1 Bioresources and biomaterials - PAD<strong>DE</strong>D VALUE PROTEIN BEVERAGE FROM WHEY VALORIZATION FORSUPPORT OF SPORTIVE EFFORTDiana PASARIN, Florin OANCEA, Camelia ROVINARU,Gheorghe RACEANU, Liliana ANTON, Lidia PREDA, Malina AVRAMInstitutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie –<strong>ICECHIM</strong>, Spl. Independentei nr. 202The purpose of this study relates to valorize of whey (resulted in large quantities frommilk processing in cheese and casein) as a 100% natural protein drinks, fortified, rehydratedand rebalanced to support sportive effort.Whey represents 85-90% of the volume of processed milk. From a production of 10million tons worldwide cheese whey results 85-90 million tones of whey with high nutritionalproperties due to its content in lactose, water, proteins, minerals, fats and vitamins. It isestimated that the food value of 3 kg whey is equivalent to that of one kg of milk, the amountof energy being 795-1046 kJ/kg, depending on the content of lactose.Whey protein has a high biological value comparative with proteins from egg, soy andcasein from milk containing branched amino acids (leucine, isoleucine, valine), whichstimulates specific intracellular pathways associated with muscle protein biosynthesis.Valorization of this product is required not only for its valuable composition, but alsobecause the discharge of whey pollutes the natural environment, biological oxygenconsumption being 50 g / l whey compared to about 0.3 g / l of waste water discharged fromurban centers. Irrigation with whey lead in time to an excessive mineralization and likeanimal feed is not economically efficient, 1kg of protein being obtained from 1.7 t whey.For valorization of whey are searched various alternatives, this by-product can be100% used, by recovery of all useful substances in its composition.Objectives proposed are: characterization of physical, chemical and microbiologicalproperties of raw materials (sweet whey, acid whey); establishing and fortifying drink recipesbased on whey, selection and implementation of advantageous variant from point of vieworganoleptic, economically and nutritionally; selection of the conservation and packagingmethod.