INSTITUTUL NAÅ¢IONAL DE CERCETARE-DEZVOLTARE - ICECHIM

3. Chemistry in medical and pharmaceutical applications - OSERINE-PROTEASE INHIBITORS FROM NATURAL SOURCES, TOACHIEVE ANTIALLERGIC PRODUCTSAuthors: Andreea COZEA, Viorica CARABELA Georgeta ALEXANDRU,Gabriel IVOPOL, Mihaela NEAGU, Natalita BORDEIS.C. HOFIGAL EXPORT IMPORT S.A. 1 Intrarea Serelor nr.2, sector 4, Bucharest, Romania, Research andDevelopment DepartamentRisk of several types of skin allergies and eczema because of a group of enzymes,serine proteases, introduced in the human body from the outside, we decided to study a,,antidote"-serine- protease inhibitors. In this case it was necessary to achieve products withthe role of inhibitors of serine-protease from vegetal material for that have been studied alarge vegetal material, especially seeds, knowing that it are the richest organs with serineproteaseinhibitors.Finally we chose flaxseed, to determine the content of serine-protease inhibitors,proteins, lipids, other possible enzymes (protease, amylase, lipase). Various methods forextractions have been applied to obtain higher values of serine-protease inhibitors and havebeen selected the best option.The complex of serine protease inhibitors was assessed byinhibition of an serine- protease, named trypsin, which was chosen as a model for the study.The kinetics of enzymatic reaction was followed by a rapid method using as substrate N-benzoyl-L-arginine-4-nitroaniline (BAPA) and inhibitory activity was determined startingfrom the diference between a trypsin solution activity and activity of the same trypsin solutionat different times after addition of inhibitor. Trypsin acts on the substrate with the formationof 4-nitroaniline that have blue color which absorbs at λ=405 nm.Based on the results obtained on the inhibition activity we studied a formula and afinished product dosage form for an external use gel. We selected plant extracts and essentialoils with therapeutic properties able to potentiate the synergism antiallergic effect of serineprotease inhibitors obtained.Bibliography1. Birk Y.1985- The Bowman-Birk inhibitor. Trypsin- and chymotrypsin-inhibitor from soybeans. Int. J. Pept.Protein Res., 25: 113-131,.2. Troll W., Frenkel K., Wiesner R.1984- Protease inhibitors as anticarcinogens. J. Natl. Cancer Inst., 73:1245-1250.