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80Justyna Szymańska et al.gle, double and triple exposure to radiation wasapplied.First five experiments (1-5) revealed 15 thousandcells within a single hole, 10 thousand in 6 th experiment,5 thousand in 7 th and 8 th experiment. There werefreshly isolated cells used in the experiments, exceptfor the 6 th sample in which the cells were previouslyfrozen. The cells of 1 st -4 th and 6 th experiment weresubjected to single irradiation. In 7 th experiment thecells were irradiated three times, two times in case of5 th and 6 th experiment. The interval between exposuresto radiation amounted to 24 hours. The laser irradiationparameters applied in the experiments are presented inTable I. Using the colorimetric detection set of livingcells, the proliferation of investigated group (subjectedto irradiation) as well as the control group was determined.The cells proliferation was denoted indirectlyby means of determining the absorption value measuredby ELISA factor.Achieved absorption values are directly proportionalto the number of living cells. The final relativepercentage values were received after correlating particularresults with mean value achieved within thecontrol group.Table 1. Laser irradiation parameters applied in the experimentsNumber Wavelenght Power Eds – Power DensityExperiment1 660 nm 40 mW 1 - 5.5 J/cm 2Experiment 2 670 nm 20 mW 0.28 – 5.71 J/cm 2Experiment 3 670 nm 20 mW 2.86 - 11.43 J/cm 2Experiment 4 670 nm 10mW i 20 mW 2.86 - 11.43 J/cm 2Experiment 5 670 nm 20 mW 2 - 8 J/cm 2Experiment 6 670 nm 10mW i 20 mW 2 - 8 J/cm 2Experiment 7 820 nm 100 mW 2 - 8 J/cm 2Experiment 8 808 nm 1500 mW 2 - 8 J/cm 2RESULTSThe experiments were to be carried out in a form ofeight examinations. There were three trials of irradiationof energy dose of 1-5.5 J/cm 2. power of 40 mWand wavelength of 660 nm introduced in the first experiment.The results are shown in Table II. It wasaffirmed that each energy dose by the constant powerof 40mW, stimulates the cells proliferation, in comparisonto the control group which was not subjected toirradiation. The most significant increase of proliferation(196.6%) was observed at the energy dose of 4.5J/cm 2. , the slightest proliferation (104%) was achievedby the energy dose of 5.5 J/cm 2 in the case of the controlgroup.Table II. Results of experiment 1Tabela II. Wyniki eksperymentu 1Parameters40 mWEds [J/cm 2 ] 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5t [s] 10 15 20 25 30 35 40 45 50 55Test 1 0.84 0.739 0.939 0.891 1.01 0.978 1.021 0.892 1.301 0.853Test 2 1.663 1.655 1.039 1.789 1.091 1.776 1.784 1.911 1.144 0.314Test 3 0.977 1.781 1.279 1.723 1.572 1.412 1.423 1.99 1.611 1.365Medium 1.16 1.392 1.086 1.468 1.224 1.389 1.409 1.598 1.352 0.844Control 1.265 0.857 0.767 0.751 0.748 0.796 0.871 0.736 0.681 0.654% 142.8 171.3 133.6 181 151 171 173.4 196.6 166.4 103.9There were five trials of irradiation with energydoses within the range of 0.28 – 5.71 J/cm 2 and powerdecreased by a half (20 mW) and wavelength of 670nm introduced in the second experiment. The resultsare shown in table III. In the case of energy dose of0.28 J/cm 2. the proliferation was not as significant as itwas noticed in the control group, which was not subjectedto irradiation. Energy doses of 1.43 J/cm 2 and5.71 J/cm 2 produced not large increase of proliferationof cells, which was more significant at the energy doseof 5.71 J/cm 2 . After decreasing power of laser irradiation,the proliferation of cells decreased significantly.Table III. Results of experiment 2Tabela III. Wyniki eksperymentu 2Parametry20 mWEds [J/cm 2 ] 0.28 1.43 5.71 Controlt [s] 5s 25s 100sTest 1 1.82 1.95 2.021 1.876Test 2 1.915 1.943 2.101 2.06Test 3 1.835 2.043 2.025 1.801Test 4 1.664 1.922 1.948 1.942Test 5 1.389 1.75 1.944 1.784Medium 1.72 1.92 2.01 1.89Control % 91.25 101.67 106.23p NS NS NSp – statistical importanceNS – non importantBasing on the results of the previous examinations,the energy intensity increased from 2.86 to 11.43 J/cm 2in the third one, with the same power as well as thewavelength. Four trials of irradiations were carried out.The results are shown in Table IV. The application ofenergy dose of 2.86 J/cm 2 caused the decrease of proliferationwith reference to the control group, which