A molecular cytogenetic analysis of chromosome behavior in Lilium ...

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Chapter 4was apparently shorter, with additional fragments present nearby (Fig. 4.1a ). This obviouslyindicated that the Asiatic chromosome was broken into two pieces.52
U-type exchanges in Lilium hybridsFig. 4.1. Chromosome breakage at metaphase I and anaphase I bridging during meiosis of interspecifichybrids of lily (006001-6). (a) a pollen mother cell with successful association showed 12 bivalents,one of which had an apparently shortened Asiatic chromosome as well as an additional fragmentnearby (white arrows); (b) FISH analysis with telomere repeat and 45s rDNA as probes revealed thepresence of a number of broken chromosomes (white arrows); (c) a dicentric bicolour bridge and anacentric bicolour fragment at anaphase I stage in one of the pollen mother cells (white arrows); (d) twounicolor bridges (green fluorescence, white arrows), as well as one bicolour bridge at anaphase I inone of the pollen mother cells; (e) two unicolour bridges at anaphase I stage in one of the pollenmother cells (blue fluorescence, white arrows); (f) anaphase I bridging, accompanied by a fragment,between two nonhomologous chromosomes from the Asiatic genome (white arrows); (g) two 45srDNA loci on the bridge indicate the bridge happened in two sister chromatids (green fluorescence,white arrows); (h) a putative ring chromosome with two telomere signals on one end and two telomeremissing on the other end (white arrow). Green fluorescence stands for chromosomes fromLongiflorum genome, and blue fluorescence represents chromosomes from Asiatic genomeFISH with telomere repeats as probe also confirmed the occurrence of chromosomebreakage at metaphase I. Normally an intact chromosome consists of two chromatids andpossesses four telomeres at meiosis, any breakage of a chromosome or chromatid can becharacterized by the absence of telomeric signals in FISH. In a few pollen mother cells atmetaphase I, a number of broken chromosomes were detected that lacked half of the telomeresignals, indicating chromosome breakage (Fig. 4.1b).Dicentric bridges and fragments at anaphase I stageBridges and fragments were detected at anaphase I and telophase I stages during meiosis. Inthe 13 genotype investigated (Fig. 4.2), diverse bridging frequencies were scored bytraditional cytogenetic observation (Table 4.1). In one genotype (006001-6), the frequency ofbridging formation reached 17%, followed by around 11% in genotype 006001-16. Mostgenotypes showed relatively low bridging frequencies (less than 10%), while two genotypes(006001-97 and 041502) didn’t show any anaphase I bridging.FISH and GISH revealed two types of bridges at anaphase I. One type of bridges andfragments involved non-sister chromatids (Fig. 4.1c). Such bridges and fragments bothshowed two different fluorescences (Fig. 4.1c). Since their variable size of the accompanyingbicolor fragments of the bicolour bridges, paracentric inversion was excluded. In addition, acommon feature of the bicolor fragments is that two fluorescences have the same lengths inall cases (Fig. 4.1c), which was also rejecting the existence paracentric inversion. This type of53
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A molecular cytogenetic analysis of
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Table of ContentsChapter 1General I
Page 8 and 9: Chapter 1LilyLilies belong to genus
Page 10 and 11: Chapter 1counterparts (Finnegan 200
Page 12 and 13: Chapter 1renewed interest in detect
Page 14 and 15: Chapter 1recombination, mechanisms
Page 16 and 17: Chapter 1is only an equational segr
Page 18 and 19: Chapter 1quite divergent with vario
Page 21 and 22: Chapter 2An assessment of chromosom
Page 23 and 24: Chromosome rearrangements in Lilium
Page 37 and 38: Chapter 3Elucidation of intergenomi
Page 39 and 40: Intergenomic recombination and chro
Page 49: Intergenomic recombination and chro
Page 52 and 53: Chapter 4AbstractMeiotic abnormalit
Page 54 and 55: Chapter 4There are some criteria to
Page 56 and 57: Chapter 4FISH experiments were perf
Page 60 and 61: Chapter 4bridges is explained as U-
Page 62 and 63: Chapter 4of the sexual polyploidize
Page 64 and 65: Chapter 4fragment have the same len
Page 66 and 67: Chapter 5AbstractSupernumerary (B)
Page 68 and 69: Chapter 5their origin, the structur
Page 70 and 71: Chapter 5Fig. 5.1. Discovery of B c
Page 72 and 73: Chapter 5In order to investigate th
Page 74 and 75: Chapter 5Centric breakage and fusio
Page 76 and 77: Chapter 5It has not, however, been
Page 78 and 79: Chapter 6The results presented in t
Page 80 and 81: Chapter 6model for molecular cytoge
Page 82 and 83: Chapter 6Fig. 6.2. The meiosis proc
Page 84 and 85: Chapter 6over events during FDR mei
Page 86 and 87: Chapter 6been detected with GISH an
Page 88 and 89: Chapter 6Another feature caused by
Page 91 and 92: ReferencesAbe, H.A., Nakano, M.N.,
Page 93 and 94: ReferencesChen, Q., and Armstrong,
Page 95 and 96: ReferencesHartlerode, A.J., and Scu
Page 97 and 98: ReferencesLarson, S.R., Kishii, M.,
Page 99 and 100: ReferencesMcClintock, B. 1931. Cyto
Page 101 and 102: ReferencesRai, R., Zheng, H., He, H
Page 103 and 104: ReferencesStewart, R.N. 1947. The m
Page 105: ReferencesZhang, L., Pickering, R.,
Page 108 and 109:
Summarychromosome rearrangements. T
Page 111 and 112:
SamenvattingLelie (Lilium) is in de
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Samenvattingaantal 35 met daarnaast
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摘要百合系百合科百
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Acknowledgements淡看世事去
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Curriculum VitaeSonglin Xie was bor
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Education Statement of the Graduate
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