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EPA Method 8000 SeriesReady-to-Inject Working Level Semi-Volatile StandardsMethod 8270C/D 5 point Semi-Volatile Calibration CurveAccuStandard provides a 5 point semi-volatile calibration curve in 2 formats. One calibration curve already incorporates the internal standards ineach level of the curve. To begin analysis, the chemist just cracks the ampule open and transfers the content to the autosampler vial. The secondsemi-volatile curve does not contain the internal standard.The analytical chemist will need to add 10 µL of internal standard to each level of the curve and the environmental samples as the vials are placedon the GC/MS. AccuStandard offers both types of curves to meet your laboratory's preference regarding the addition of internal standards.Method 8270Target Analytes (Semi-Volatiles)AcenaphtheneAcenaphthyleneAnilineAnthraceneAzobenzeneBenz[a]anthraceneBenzidineBenzo[b]fluorantheneBenzo[k]fluorantheneBenzoic acidBenzo[g,h,i]peryleneBenzo[a]pyreneBenzyl alcohol4-Bromophenyl phenyl etherButyl benzyl phthalateCarbazole4-Chloroanilinebis(2-Chloroethoxy)methanebis(2-Chloroethyl)etherbis(2-Chloro-1-methylethyl)ether4-Chloro-3-methylphenol2-Chloronaphthalene2-Chlorophenol4-Chlorophenyl phenyl etherChryseneo-Cresolp-CresolDibenz[a,h]anthraceneDibenzofuranDi-n-butyl phthalate1,2-Dichlorobenzene1,3-Dichlorobenzene1,4-Dichlorobenzene3,3'-Dichlorobenzidine2,4-DichlorophenolDiethyl phthalate2,4-DimethylphenolDimethyl phthalate4,6-Dinitro-2-methylphenol2,4-Dinitrophenol2,4-Dinitrotoluene2,6-DinitrotolueneDi-n-octyl phthalatebis(2-Ethylhexyl)phthalateFluorantheneFluoreneHexachlorobenzeneHexachlorobutadieneHexachlorocyclopentadieneHexachloroethaneIndeno[1,2,3-cd]pyreneIsophorone2-MethylnaphthaleneNaphthalene2-Nitroaniline3-Nitroaniline4-NitroanilineNitrobenzene2-Nitrophenol4-NitrophenolN-NitrosodimethylamineN-NitrosodiphenylamineN-Nitrosodi-n-propylaminePentachlorophenolPhenanthrenePhenolPyrenePyridine1,2,4-Trichlorobenzene2,4,5-Trichlorophenol2,4,6-TrichlorophenolSurrogates AnalytesPhenol-d 2-FluorobiphenylNitrobenzene-d 5 2,4,6-Tribromophenol2-Fluorophenol5p-Terphenyl-d 14Technical NoteAccuStandard has prepared both Semi-Volatilecurves from special high concentration stocks. Theuseful shelf life for these 5 point curves is similar tothe shelf life experienced at the environmentallaboratory when the standard is prepared frompreviously used high concentrate stocks.Internal Standard AnalytesAcenaphthene-d 10Chrysene-d 121,4-Dichlorobenzene-d 4Naphthalene-d 8Perylene-d 12Phenanthrene-d 10Working Level Semi-Volatiles CurveWith Internal StandardsM-8270-CAL-IS-SETAll solutions in CH 2 Cl 2$ 275 / 5 x 1 mL83 comps.Components Level 1 Level 2 Level 3 Level 4 Level 5(units in µg/mL) ( 2.5X ) ( 4X ) ( 6X ) ( 8X )Target Analytes 20 50 80 120 160Surrogate Analytes 20 50 80 120 160Internal Analytes 40 40 40 40 40Level 2 Daily QC Working LevelCCC (with Internal Standard)M-8270-IS-WL-2.5X-5ML$ 275 / 1 x 5 mLM-8270-IS-WL-2.5X-10ML$ 425 / 1 x 10 mLAt stated conc. in CH 2 Cl 2Working Level Semi-Volatiles CurveWithout Internal StandardsM-8270-CAL-SETAll solutions in CH 2 Cl 2$ 250 / 5 x 1 mL77 comps.Level 2 Daily QC Working LevelCCC (without Internal Standard)M-8270-WL-2.5X-5ML$ 250 / 1 x 5 mLM-8270-WL-2.5X-10ML$ 400 / 1 x 10 mLComponents Level 1 Level 2 Level 3 Level 4 Level 5(units in µg/mL) ( 2.5X ) ( 4X ) ( 6X ) ( 8X )Target Analytes 20 50 80 120 160Surrogate Analytes 20 50 80 120 160At stated conc. in CH 2 Cl 2246 AccuStandard is accredited to ISO/IEC 17025 and certified to ISO 9001

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