Theoretical and Experimental DNA Computation (Natural ...

132 5 Physical Implementations
Fig. 5.12. Visualization of gel resulting from Experiment 20
Lanes 4 to 6 show the result of the removal of strands encoding v2 = green.
Lane 5, corresponding to the v2 = green primer, is empty, showing that no
strands containing that sequence were present. Lanes 4 and 6, corresponding
to v2 = red and v2 = blue primers respectively contain normal length product,
showing that strands not containing the sequence v2 = green were not
removed.
Lanes 7 to 9 show the result of the removal of strands encoding v2 = blue.
Lane 9, corresponding to the v2 = blue primer is empty, showing that no
strands containing that sequence were present. Lanes 7 and 8, corresponding to
v2 = red and v2 = green primers respectively contain normal length product,
showing that strands not containing the sequence v2 = blue were not removed.
The streaks visible at 74 and 18 b.p. are due to the presence of primer
dimers and free primers respectively.
Table 5.4. Interpretation of Fig. 5.12
Lane Excluded PCR Primer Result
1 v2 =red +
2 v2 =red v2 = green +
3 v2 =blue +
4 v2 =red +
5 v2 = green v2 = green -
6 v2 =blue +
7 v2 =red +
8 v2 =blue v2 = green +
9 v2 =blue -
10 None v2 =red -
11 (PCR -ve v2 = green -
12 control) v2 =blue -