102 Tea fungus synthesized bacterial cellulose
Weiterführende Techniken 103 The invention discloses a tea fungus synthesized bacterial cellulose pressure sore dressing as well as a preparation method and the application thereof. The tea fungus synthesized bacterial cellulose pressure sore dressing is a tea fungus synthesized bacterial cellulose membrane, and the cellulose component is mainly formed by secretion and synthesis of tea fungus strains in the fermentation culturing process in a sugar tea culture medium containing carboxymethyl chitosan; the water absorption rate of the dressing is 89-94.3%, the porosity is 85-91.2%, and the water vapor transmission rate is between 1,383.5 g.m.d and 1,486.5 g.m.d. According to the invention, since the nano bacterial cellulose pressure sore dressing is prepared with a sugar tea fermentation method by adopting the tea fungus, the method for synthesis of bacterial cellulose has the advantages of low cost, low environment requirement for synthesis sites and simple technical requirements; the nano bacterial cellulose material has favorable biosecurity and histocompatibility, which cannot cause stimulation and sensitization to a wound surface, and is conducive to the promotion of wound healing. A synthesis of the bacterial cellulose Kombucha pressure sores dressing, characterized in that: a Kombucha synthetic bacterial cellulose membrane, cellulose component mainly composed of Kombucha strains containing carboxymethyl chitosan sugar tea culture The fermentation process of secretion synthesis; 89~94.3% water absorption of the dressing, 85~91.2% porosity, the water vapor transmission rate of 1435 ± 51.5 _2 1-1g.m.α ο 2.- kinds of bacterial cellulose synthesis Kombucha pressure sores dressing preparation, characterized by: comprising the steps of: Cl) in accordance with the percentage by weight containing 0.5 ^ 2 percent of black or green tea, 1 (Γ20% sucrose or glucose was added distilled water containing 500mL beaker, boil 5 to 15 minutes, cooled to room temperature to obtain a solution I; (2) the above step (I) prepared solution I was filtered, the residue after removal of the tea leaves were added relative by weight or volume percentage in the final contents were 0.5~lwt%, 0.5~1% ν / ν, 0.5~lwt% and 0.5~lwt% of yeast, alcohol, carboxymethyl chitosan and peptone, stirring to dissolve, too Solution II; (3) in the above step (2) was II by double-distilled water, f 5% of the total volume of acetic acid solution and PH value is adjusted separately and 4.5~5.5 IL, pasteurization sterilization 20 ~ 30 minutes; (4) The above steps basal medium (3) is poured into a freshly prepared to fly with 3 layers of clean gauze, clean container ventilation, adding kombucha mother plant, 30 ° C temperature, clean environment 7 to 10 days under stationary culture; (5) In achieving the above step incubation time (4), the gas in the medium - liquid interface translucent, gelatinous film carefully removed, the film is Kombucha synthetic bacterial cellulose membrane, the Kombucha synthetic bacterial cellulose film was rinsed with distilled water three times, each time 1 (Γ15 minutes to remove the residual impurities media and film; (6) The above steps Bacterial cellulose membrane (5) in the gel was immersed in synthetic `Kombucha molar concentration of 0.05, NaOH solution .1M in boiling 15 ~ 20 minutes to further remove the residual bacterial membrane components and other impurities; (7) The kombucha synthetic bacterial cellulose membrane (6) tre-