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Presentation - University of Colorado Denver

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Composition<br />

•Calories<br />

oLactose<br />

oProtein<br />

oFat<br />

•Glucose<br />

•Fatty Acid<br />

Pr<strong>of</strong>ile*<br />

•Free Fatty Acids<br />

•Triglycerides<br />

Composition<br />

• Glucose<br />

• RBC Membrane Fatty<br />

Acid Composition*<br />

• Free Fatty Acids<br />

• Triglycerides<br />

• Hb-A1C<br />

Breast Milk (6 samples/Participant = 480 total samples)<br />

Cytokines<br />

•IL-6*<br />

•IL-8*<br />

•IL-10*<br />

•TNF-α*<br />

•IFN-γ*<br />

•CRP<br />

Cytokines<br />

•IL-6*<br />

•IL-8*<br />

•IL-10*<br />

•TNF-α*<br />

•IFN-γ*<br />

•CRP<br />

Biochemical Analyses<br />

Hormones &<br />

Adipokines<br />

•Insulin<br />

•Leptin<br />

•Adiponectin<br />

•Ghrelin (total)<br />

•Ghrelin (acylated)<br />

Maternal Blood (2 Samples/Participant = 160 total samples)<br />

Hormones &<br />

Adipokines<br />

•Insulin<br />

•Leptin<br />

•Adiponectin<br />

•Ghrelin (total)<br />

•Ghrelin (acylated)<br />

Antioxidants Microbiota<br />

• Total antioxidant • Probiotic Content*<br />

capacity<br />

• DPH Radical<br />

Scavenging Activity<br />

• Catalase Activity<br />

Oxidative Stress<br />

•F2-Isoprostanes<br />

•Oxidized-LDL<br />

•TBARS<br />

•8-OH-dG<br />

•4-HNE<br />

Infant Urine and Stool (2 Samples/Participant = 160 total samples)<br />

Oxidative Stress (urine)<br />

•F2-Isoprostanes<br />

Oxidative Stress<br />

•F2-Isoprostanes<br />

•Oxidized-LDL<br />

•TBARS<br />

•8-OH-dG<br />

•4-HNE<br />

Microbiota (stool)<br />

• Intestinal Microbiome Pr<strong>of</strong>ile*<br />

* All cytokines except CRP will be analyzed on a multiplex panel, microbiota analyses, fatty acid pr<strong>of</strong>ile, and<br />

membrane fatty acid composition will be analyzed in the labs <strong>of</strong> Dr. Daniel Frank and Dr. Mary Harris, respectively.<br />

All other analyses can be undertaken in our lab, but analytes highlighted in purple are <strong>of</strong>fered by the CTRC core<br />

labs.

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