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official journal of the patent office - Controller General of Patents ...

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(12) PATENT APPLICATION PUBLICATION (21) Application No.00178/DELNP/2003 A<br />

(19) INDIA<br />

(22) Date <strong>of</strong> filing <strong>of</strong> Application :17/02/2003 (43) Publication Date : 16/03/2007<br />

(54) Title <strong>of</strong> <strong>the</strong> invention : A RAPID METHOD FOR MICROWAVE MEDIATED ENZYME-LINKED<br />

IMMUNOSORBENT ASSAY<br />

(51) International classification :G01N 33/543 (71)Name <strong>of</strong> Applicant :<br />

(31) Priority Document No :PCT/IN00/00075 1)COUNCIL OF SCIENTIFIC AND INDUSTRIAL<br />

(32) Priority Date :16/08/2000 RESEARCH<br />

(33) Name <strong>of</strong> priority country :India<br />

Address <strong>of</strong> Applicant :RAFI MARG, NEW DELHI-<br />

(86) International Application No :PCT/IN00/00075 110001, INDIA Delhi India<br />

Filing Date<br />

:16/08/2000 (72)Name <strong>of</strong> Inventor :<br />

(87) International Publication No :WO 02/14868 1)PRADEEP NAHAR<br />

(61) Patent <strong>of</strong> Addition to Application<br />

Number<br />

Filing Date<br />

:NA<br />

:NA<br />

2)UTPAL BORA<br />

3)GAINDA LAL SHARMA<br />

(62) Divisional to to Application<br />

Number<br />

Filing Date<br />

:NA<br />

:NA<br />

No <strong>of</strong> Pages : 32 No <strong>of</strong> Claims : 15<br />

(57) Abstract :<br />

This invention relates to a rapid and efficient method for carrying out enzyme-linked immunosorbent assay for<br />

detection <strong>of</strong> minute quantities <strong>of</strong> biomolecules such as antigen, antibody etc. This invention particularly relates to<br />

microwave mediated immobilization <strong>of</strong> antigen or antibody on to <strong>the</strong> activated surface followed by performing<br />

subsequent steps <strong>of</strong> ELISA by controlled microwave irradiation. The invented procedure has dramatically reduced <strong>the</strong><br />

total time required for ELISA to less than 10 minutes from hours to days. The invented ELISA procedure is rapid,<br />

economical, reproducible and simple and can be automated. The invented procedure is useful for carrying out ELISA<br />

in clinical diagnostics, molecular biology, agriculture, sericulture, food technology, environmental science,<br />

biomedical research and o<strong>the</strong>r related fields.<br />

The Patent Office Journal 16/03/2007 5504

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