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Adil GÜNER, Vehbi ESER - optima

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VEGETATIVE ANATOMY OF Orchis italica Poiret (ORCHIDACEAE)<br />

IN TURKEY<br />

Ernaz ALTUNDA� 1 , Ece SEVG� 2<br />

1 Duzce University, Faculty of Arts and Sciences, Departrment of Biology, Düzce, Turkey<br />

ernazaltundag@duzce.edu.tr<br />

2 Do�anbey Cad. Me�e sok. Öznur Apt. No. 1 D: 3, Bahçeköy, �stanbul, Turkey<br />

ecesevgi1@yahoo.com<br />

Anatomical characteristics of the vegetative parts of Orchis italica Poiret in Turkey have been<br />

investigated in this study. Orchis italica samples were collected from Ezine (Çanakkale),<br />

Selçuk (�zmir) and Milas (Mu�la) between 2007 and 2009. Permanent microscopic<br />

preparations were made of plant material fixed in field in 70 % alcohol. Cross sections of the<br />

leaves, stem, tuber and root and surface sections of leaves taken by free-hand and stained with<br />

Sartur solution and Safranin. The well-staining sections were photographed on Leica DFC295<br />

color camera type, Leica DM2500 light microscope.<br />

The leaves are anomostomatic and tetrastic, have no trichomes. The cuticle thickness (abaxial<br />

and adaxial), epidermis cell size (abaxial and adaxial) and stomata dimensions and stomata<br />

index were measured. The epidermis are lined parellel by the midrib and surrounded by<br />

cuticle. In cross sections of the lamina, upper epidermis are larger than lower epidermis.<br />

Vascular bundles are collateral and consist of xylem, phloem and sclerenchyma cells. Raphide<br />

are observed in the mesophyll tissue. Midrib has lacunas. Chlorenchyma has been scattered<br />

homogeneous. In cross sections of stem, the epidermis consist of a single layered, flattened,<br />

roundish or ovate cells and surrounded by thin cuticle layer. Parenchyma and chlorenchyma<br />

were observed along cortex. Vascular bundles are collateral type and are surrounded by<br />

sclerencymatic tissue. In cross sections of the tuber, parenchymatic pith cells have starch and<br />

raphide. A single layered epidermis, exodermis, parenchymatic cortex, a single layered<br />

endodermis, periscyle, and vascular bendle have been observed in cross sections of the root.<br />

Keywords: Orchis italica, Orchidaceae, leaf anatomy, stem anatomy, tuber anatomy, root<br />

anatomy, raphide.<br />

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