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Posters
90
TAXONOMY OF COLLETOTRICHUM SPECIES COLLECTED FROM
IRAN
Doustmorad ZAFAR�
Bu Ali Sina university, Dept. Plant protecton, Hamedan. Iran; Zafari_d@basu.ac.ir
In this study in order to collect different Colletotrichum species, different samples were
collected from different crops especially from potato fields of Hamedan and Lorestan
provinces. Samples were cultured on PDA medium and Colletotrichum species were isolated.
The isolates were distinguished based on morphological characteristics including color of
colonies, conidial and appressorial shape and size and presence or absence of setae and
sclerotia. In this study 9 species and one variety of the genus Colletotrichum were identified
including C. acutatum, C. boninense, C. coccodes, C. dematium, C. destructivum,
C. gloeosporioides, C. gloeosporioides var. minus, C. musae, C. trichellum and C. truncatum.
The sexual stages of some of the isolates such as C. boninense were produced in vitro. The
teleomorph of C. boninense hadn't been reported before and was introduced as Glomerella sp.
in this study. C. coccodes is the casual agent of potato black dot and was isolated from all of
the samples from potato fields of Hamedan province. Potato black dot is the most important
Colletotrichum disease in Hamedan province and its significance has been increased in recent
years. The ITS regions (ITS1, ITS2 and 5.8S gene) of ribosomal DNA of 26 different isolates
including 9 C. coccodes isolated were sequenced in order to examine the accuracy of the
morphological identifications and to compare C. coccodes isolates. ITS sequences of
C. coccodes isolates were studied separately and no genetic diversity was seen among them
according to ITS sequences. Therefore the sequences of these regions are not useful for
determining intraspecific diversities, whereas ITS sequences in other Colletotrichum species
especially in ITS1 was very divergent and they are a good marker for distinguishing
Colletotrichum species. Phylogenetic analysis based on ITS sequences of Colletotrichum
species of this study and the ones obtained from gene bank, the species are divided into 6
main clades. The separation of these clades is compatible with morphological characteristics
specially conidial shape. Among the identified Colletotrichum species in this study, the
species C. acutatum, C, boninense, C. destructivum and the variety C. gloeosporioides
var. minus are new for the mycoflora of Iran and the species C. dematium isolated from bean,
C. gloeosporioides from ivy arum and C. truncatum from alfalfa are isolated for the first time
from these hosts.
Keywords: Colletotrichum, anthracnose, ITS
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