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Adil GÜNER, Vehbi ESER - optima

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PHYLOGENETIC RELATIONSHIPS OF TAXA OF DISSITIFLORI DC.<br />

SECTION IN GENUS ASTRAGALUS L. USING SEED STORAGE<br />

PROTEIN POLYMORPHISM<br />

Handan �APÇI¹, Dilek DABANLI¹, Servet ÖZCAN², Murat EK�C�³, Cem VURAL², Hasan<br />

AKAN 4<br />

¹Graduate School of Natural and Applied Sciences, Erciyes University, Kayseri, Turkey handan@gmail.com<br />

²Department of Biology, Faculty of Arts and Science, Erciyes University, Kayseri, Turkey vuralc@erciyes.edu.tr<br />

³Department of Biology, Faculty of Art and Sciences, Gazi University, Ankara, Turkey mekici@gazi.edu.tr<br />

4 Department of Biology, Faculty of Art and Sciences, Harran University, �anl�urfa, Turkey hakan@harran.edu.tr<br />

The genus Astragalus are widely distributed in Turkey and have various taxonomic problems.<br />

The aim of this present work was to study the phylogenetic relationships of the taxa of<br />

Dissitiflori section in genus Astragalus. Unique pattern of protein bands of total seed storage<br />

protein obtained from twelve taxa was investigated by using sodium dodecil sulphatepolyacrylamid<br />

gel electrophoresis (SDS-PAGE). Phylogenetic relationships were displayed in<br />

NJ (Neighbor-joining) tree. The taxa were grouped on two main clads. The first clad<br />

composed of A. kastamonuensis D.F. Chamb. & V.A. Matthews and A. nigrifructus Podlech<br />

& Aytaç; and second clad composed of A. beypazaricus Podlech & Aytaç, A. nitens Boiss. &<br />

Heldr., A. gladiatus Boiss., A. subulatus Pall., A. taochius Woronov, A. argyroides Beck, A.<br />

cornutus Pall., A. viridis Bunge, A. aucheri Boiss. and A. leptothamnus Bunge. According to<br />

the matrix of genetic distance, the lowest values were between A. aucheri and A.<br />

leptothamnus (0.071). From genetic distance value and NJ tree, it may be concluded that A.<br />

aucheri and A. leptothamnus belong to same species. Our findings from this study indicated<br />

that seed storage protein profiles could be useful markers in studies of phylogenetic<br />

relationships of the genus Astragalus.<br />

Keywords: Astragalus, Dissitiflori, SDS-PAGE, seed storage protein, phylogenetic analysis<br />

117<br />

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