Adil GÜNER, Vehbi ESER - optima

ISSR ANALYSES ON GENUS FIBIGIA MEDIK. (BRASSICACEAE) IN
TURKEY
Özlem ÇET�N 1 , Ahmet DURAN 1 , Erdo�an E. HAKKI 2
1
Selçuk Üniv. A.K. E�itim Fak. Biyoloji E�itimi, Meram, Konya ozlemcetin8419@gmail.com,
ahmetduran22@yahoo.com
2
Selçuk Üniv. Ziraat Fak. Tarla Bitkileri Bölümü, Konya eehakki@yahoo.com
Abstract: Fibigia Medik (Brassicaceae) genus is distributed in Southern Europe, Caucasus,
Middle East, Egypt and represented by almost 16 taxa in the world. The four taxa of the genus
are available in Turkey. In this study, Fibigia species were collected from different localities
in Turkey. Genomic DNA was isolated from silica gel dried leaves following the procedure of
Doyle & Doyle (1987). Out of 22 primers used in preliminary tests, 10 most suitable ones (in
terms of repeatability, scorability and the ability to distinguish between species) were selected
for identification. The amplified fragments were scored for markers being either present (1) or
absent (0). The molecular connection of the Fibigia taxa with the Physoptychis, Bornmuellera
and Aurinia genus was studied and their phylogenetic relations were revealed with the
dendrogam which was prepared by the NTSYSpc 2.1 software.
Keywords: Fibigia, Brassicaceae, ISSR, NTSYS, Turkey
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