Adil GÜNER, Vehbi ESER - optima

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Posters 88 PHYLOGENETIC RELATIONSHIPS OF TAXA OF HYPOGLOTTIDEI DC. SECTION IN GENUS ASTRAGALUS L. USING SEED STORAGE PROTEIN POLYMORPHISM Dilek DABANLI¹, Handan �APÇI¹, Murat EK�C�³, Servet ÖZCAN², Cem VURAL², Hasan AKAN 4 ¹Graduate School of Natural and Applied Sciences, Erciyes University Kayseri, Turkey; handan@gmail.com ²Department of Biology, Faculty of Arts and Science, Erciyes University, Kayseri,Turkey; vuralc@erciyes.edu.tr ³Department of Biology, Faculty of Art and Sciences, Gazi University, Ankara, Turkey; mekici@gazi.edu.tr 4 Department of Biology, Faculty of Art and Sciences, Harran University, �anl�urfa,Turkey; hakan@harran.edu.tr The genus Astragalus are widely distributed in Turkey and have various taxonomic problems. The aim of this present work was to study the phylogenetic relationships of the taxa of Hypoglottidei section in genus Astragalus. Unique pattern of protein bands of total seed storage protein obtained from seventeen taxa was investigated by using sodium dodecil sulphate-polyacrylamid gel electrophoresis (SDS-PAGE). Phylogenetic relationships were displayed in NJ (Neighbor-joining) tree. The taxa were grouped on two main clads. The first clad composed of A. melanocarpus Bunge, A. hartvigii Kit Tan., A. saganlugensis Trautv. and A. akmanii Aytaç & H. Duman; and second clad composed of A. vexillaris Boiss., A. scholerianus Bornm., A. ovatus DC., A. viridissimus Freyn & Sint., A. dasycarpus D.F. Chamb., A. cedreticola A. Duran & Podlech, A. lasioglottis M. Bieb, A.oreades C.A. Mey, A. viciaefolius D.C., A.sachanewii Sirj., A.bachmarensis Grossh., A. cicer L. and A. flaccidus M. Bieb. According to the matrix of genetic distance, the lowest values were between A. viciaefolius and A. flaccidus (0.125), A. viciaefolius and A. sachanewii (0.167), A. dasycarpus and A. sachanewii (0.194). From genetic distance value and NJ tree, it may be concluded that A. flaccidus and A. viciaefolius belong to same species and similarly A. dasycarpus and A. sachanewii as well. Our findings from this study indicated that seed storage protein profiles could be useful markers in studies of phylogenetic relationships of the genus Astragalus. Keywords: Astragalus, Hypoglottidei, SDS-PAGE, seed storage protein, phylogenetic analysis 24
A NUMERICAL TAXONOMIC STUDY IN THE TAXA OF GENUS NIGELLA L. (RANUNCULACEAE) Dilek ÖZÇEL�K 1 , Ali A. DÖNMEZ 1 , Zübeyde U�URLU 1 1 Department of Biology, Faculty of Science, Hacettepe University, 06800 Beytepe, Ankara, Turkey; a.dilek.ozcelik@gmail.com; zubeydeugurlu@gmail.com; donmez@hacettepe.edu.tr A monographic study of the genus Nigella s.l. is ongoing by the authors. This numerical taxonomic study is based on about 155 specimens of Nigella collected from the field in Turkey, Syria, the Turkish Republic of Northern Cyprus and Greece or from the material deposited at various herbaria. The measurments and observations have been made on 67 morphological characters and they have been studied for 22 taxa as operational unit (OTU). The data were analysed with the NTSYSpc package program for understanding similarities among the OTU’s. According to the obtained phenogram, the taxa belonging to the genera Garidella and Komaroffia are clearly distinct from the taxa of Nigella. The taxa belong to the N. arvensis complex are not clearly different from each other with higer similarity index. The complex requires further taxonomic and nomenclatural studies for resolving the relationship of them. Yellow flowered species of the genus are also close to each other the results according to this study. Keywords: Nigella, phenogram, Ranunculaceae, taxonomy MOLECULAR PHYLOGENETICS AND GENOME SIZE VARIATION IN VERBASCUM (SCROPHULARIACEAE) Dirk C. ALBACH Carl von Ossietzky-University, Institute of Biology and Environmental Sciences, Oldenburg, Germany dirk.albach@uni-oldenburg.de The genus Verbascum with approximately 350 species is one of the largest genera of Turkey with about 235 species, 80% endemic. Possible reasons for the high estimate of endemicity are successful adaptation to the dry, Mediterranean climate, diverse topography, variability in life history (annual, biennial, perennial) and a propensity for hybridization. The importance of each one of these factors is unknown. In a first step to unravel the success of Verbascum, I started a DNA-based phylogenetic analysis for the genus. For this purpose herbarium material was carefully checked and appropriate material collected for 120 species. Initial screening of nuclear ITS sequences and sequences of two cpDNA regions revealed first incongruencies. Results will be discussed in the light of existing taxonomic hypotheses for the genus and morphological characters. One character that will be investigated in parallel to the phylogenetic analysis is genome size evolution. Genome size is indicative of ploidy changes but also associated with breeding system and possibly life history variation. Intrageneric variation so far detected is low and does not support an influence of life history. Keywords: cpDNA, genome size, ITS, Verbascum 89 25 Posters
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OrganizationCommittee: ............
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) Posters: Ahmet AKSOY, Durmu� OK
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H. A�k�n AKPULAT, Ebru ATA�LA
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Stanislav ŠPANIEL, Judita LIHOVÁ,
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March 22, 2010 Monday 09:00 - 10:30
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11:45 - 12:15 AKAN, H. "The Botanic
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Oral Lectures 14 CONSERVATION OF ME
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Oral Lectures METALLOPHYTES, HYPERA
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Oral Lectures 18 STUDY CASES ON CON
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Oral Lectures 20 SYSTEMATICAL STUDI
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Oral Lectures 22 BOTANICAL COLLECTI
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Oral Lectures 24 REVISION OF THE GE
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Oral Lectures 26 PROPOSALS FOR A RE
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Oral Lectures 28 BIOGEOGRAPHICAL LI
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Oral Lectures 30 ETHNOBOTANY IN ANA
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Oral Lectures 32 POLLINATION ECOLOG
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Oral Lectures 34 WHY IS THERE A WOR
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Posters 142 POLLINATION SUCCESS OF
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Posters THE TAXA OF CR AND EN CATEG
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Posters 148 THE ENDEMIC PLANTS OF D
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Posters 150 THE GENETIC DIVERSITY O
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Posters A SYNECOLOGICAL AND SYNTAXO
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Posters INTROGRESSIVE HYBRIDIZATION
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Posters COMPONENTS OF POLLINATOR EF
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Posters 160 ANATOMY, TRICHOME AND N
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Posters 162 THE TURKISH GEOPHYTE CO
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Posters 180 ELEMENTAL ANALYSES OF A
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Posters 182 REAL AND POTENTIAL UPPE
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Posters CONSERVATION OF BIODIVERSIT
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Adil GÜNER, Vehbi ESER - optima

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