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Posters
96
PRELIMINARY MOLECULAR CYTOGENETICS STUDIES OF
CROCUS FROM BOLU IN TURKEY
Emel USLU, Mehmet Tekin BABAÇ, �smail EKER
Abant �zzet Baysal University, Faculty of Science and Arts, Biology Department, Gölköy, Bolu
emel.uslu@gmail.com
Natural distributed Crocus taxa in Bolu provience were used to attempt for the application of
molecular studies, such as, “genomic in situ hybridization (GISH)”. This method allows
chromosomes from different parents or ancestors to be distinguished by means of differential
hybridization of entire genomic probes. It has been aimed that application of this technique on
some Crocus taxa and to be diagnosed any hybrid origins. Beside these molecular studies,
karyotypes of those Crocus taxa have also been prepared.
In order to identify, whether there is any hybrid among those Crocus taxa, genomic DNA of
each taxon (C. abantensis T. Baytop et Mathew, C. ancyrensis (Herbert) Maw, C. biflorus
Miller subsp. pulchricolor (Herbert) Mathew, C. olivieri Gay subsp. olivieri Gay, C. specious
Bieb. subsp. specious) was labelled by fluorescein and applied the chromosomes of others.
Although, there was no hybridization signal detected among these taxa, the known hybrid
Crocus x paulineae Pasche & Kerndorff taxon gave signal with C. olivieri subsp olivieri and
C. abantensis. The application of method was succeded and therefore, similar studies can be
applied on to different species to identify their parental origins.
Keywords: Bolu, Crocus, GISH, Karyotype, Molecular cytogenetics
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