Adil GÜNER, Vehbi ESER - optima
Adil GÜNER, Vehbi ESER - optima
Adil GÜNER, Vehbi ESER - optima
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GENOME ORGANIZATION OF SOME MEDITERRANEAN GEOPHYTES<br />
- FACULTATIVE SERPENTINOPHYTES<br />
Fatima PUSTAHIJA 1,2,3 , Edina MURATOVIC 3 and 1 Sonja SILJAK-YAKOVLEV 1,3<br />
1CNRS, Université Paris-Sud, AgroParisTech, UMR 8079, Ecologie, Systématique, Evolution, Départ. "Biodiversité,<br />
Systématique et Evolution", Bât. 360, 91450 Orsay, France ; fatima.pustahija@u-psud.fr : sonia.yakovlev@u-psud.fr<br />
2 Faculty of Forestry, University of Sarajevo, Zagreba�ka 25, 71000 Sarajevo, Bosnia and Herzegovina<br />
3 Department of Biology, University of Sarajevo, Faculty of Sciences, Laboratory for research and protection of<br />
endemic resources, Zmaja od Bosne 33, 71000 Sarajevo, Bosnia and Herzegovina<br />
edina1muratovic@yahoo.com<br />
In this work we report the changes of the genome organization in some serpentine populations for<br />
three lilies species (Lilium albanicum, L. bosniacum and L. martagon) and Poet’s daffodil<br />
(Narcissus poeticus L.), in comparison with other populations growing in <strong>optima</strong>l conditions in<br />
the region of Dinaric Alps. The karyotype and chromosomal organization were analyzed by<br />
fluorochrome banding (chromomycin A3 and DAPI) and double Fluorescence In Situ<br />
Hybridization (FISH) using 18S-5.8-26S and 5S rDNA probes. It was observed that populations<br />
from stressful and not <strong>optima</strong>l environment conditions such as atypical serpentine substrate<br />
possessed B chromosomes bearing rDNA genes. It was confirmed that in all atypical populations<br />
the presence of one or two CMA positive bands on B chromosomes corresponded to 18S, or<br />
sometimes to 5S rDNA loci (Lilium bosniacum). These supplementary 18S loci at B<br />
chromosomes are probably active, since the increased number of nucleoli was observed.<br />
This study supports the hypothesis that superior plants surviving in atypical natural habitats<br />
frequently generate B chromosomes which can present some adaptive advantages. Furthermore, it<br />
provides opportunity for complex investigations of possible role of B chromosomes.<br />
Keywords: Narcissus, Lilium, B chromosomes, heterochromatin pattern, rDNA organization<br />
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