2. Behavioral Biology TALKS - Deutsche Zoologische Gesellschaft
2. Behavioral Biology TALKS - Deutsche Zoologische Gesellschaft
2. Behavioral Biology TALKS - Deutsche Zoologische Gesellschaft
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y predation risk. However, hunger level affected kin preferences depending on the<br />
body size of the test fish. Small, hungry juveniles avoided to shoal with kin whereas<br />
small, saturated juveniles as well as larger juveniles in general tended to<br />
preferentially shoal with kin. We discuss the results in the context of size-related<br />
energetic demands.<br />
����107 Arndt Wellbrock<br />
To bleed or not to bleed? - Validation of buccal swabs compared to blood as DNA<br />
source for molecular sexing of birds<br />
Author: Arndt Wellbrock 1 , Christina Bauch 1 , Jan Rozman 1 , Klaudia Witte 1<br />
Affiliation: 1 Research Group on Ecology and <strong>Behavioral</strong> <strong>Biology</strong>, Section of <strong>Biology</strong> and<br />
Didactics, Department of Chemistry - <strong>Biology</strong>, University of Siegen<br />
Buccal swabbing is an easy, non-invasive and alternative method to blood sampling<br />
to get DNA for sexing birds. However, little is known about the reliability of this<br />
sampling method. Here, we investigated the reliability of buccal swabs for molecular<br />
sex determination in very young nestlings (0 to 14 days old) and adults of the<br />
Common Swift (Apus apus) by comparing sex determination based on buccal swabs<br />
and based on blood samples of the same individuals. Thereby, buccal swabbing was<br />
tested for sexing a small non-passerine bird for the first time. We found that buccal<br />
swabs are a reliable non-invasive source of DNA for sex determination in both adult<br />
and very young swifts. In 47 out of 53 (89%) nestlings and 10 out of 11 (91%) adults,<br />
we received enough DNA via buccal swabs for a successfull PCR. Sex determination<br />
based on DNA from buccal swabs matched the result of sex determination based on<br />
blood samples in 46 of the 47 nestlings (98%) and in all 10 cases in adults (100%<br />
matching). Since PCR amplification was performed only once per individual, we<br />
consider this success rate - and hence the reliability of buccal swabs as DNA source -<br />
as very high. Therefore, we recommend buccal swabs as a feasible non-invasive<br />
method in future genetic studies in birds, especially for small nestlings and in species<br />
which are sensitive to stress.<br />
����108 Mareike Wurdack<br />
Consequences of an arms race between a host and its three brood parasites<br />
Authors: M. Wurdack 1 , J. Kroiss 1 , E. Strohm 1 , O. Niehuis 1 , T. Schmitt 1<br />
Affiliation: 1 Universität Freiburg<br />
Parasites and hosts have conflicting interests ? to either exploit or defend. This sets<br />
the board for an evolutionary arms race. The species pair follows a trajectory through<br />
repeated cycles of fine tuning of the parasite?s attack strategies and evasive actions<br />
of the host. Brood parasites in particular need to avoid detection by the host. Insect<br />
brood parasites may avoid olfactory detection by mimicking the host?s cuticular<br />
hydrocarbon (CHC) profile. The arms race would then lead to optimized chemical<br />
mimicry in the parasite. The host could e.g. escape a mimetic match by changing its<br />
CHC composition.<br />
Ordinary parasitic associations consist of one enemy (the parasite) and one target<br />
(the host). But more complex variations are possible, too. In this study, a solitary host<br />
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