2. Behavioral Biology TALKS - Deutsche Zoologische Gesellschaft

����166 Martin Strauch
ImageBee - A KNIME plugin for analysing calcium-imaging data from the honeybee
antennal lobe
Authors: Martin Strauch 1 , C. Giovanni Galizia 1
Affiliation: 1 Universität Konstanz, Neurobiologie
Calcium-imaging with fluorescent dyes is an established technique for recording
glomerular odor response patterns in the insect antennal lobe. Here we present
ImageBee [1], a software for analysing calcium-imaging recordings from the
honeybee antennal lobe.
ImageBee is available as a plugin for the modular data analysis platform KNIME
(www.knime.org) that provides a user-friendly graphical interface for constructing
signal processing and data analysis pipelines.
Based on the algorithm presented in [2], ImageBee contains the tools to
automatically construct functional maps (segmentations) of the antennal lobe, to
compute glomerular signals from imaging movies, and to construct denoised versions
of the movies.
Preprocessing methods such as spatial and temporal filtering or motion correction
are available, and the modular principle of KNIME allows for future extensions of the
toolkit, also for application to imaging data from other insects.
ImageBee provides a solid foundation for data analysis in insect brain imaging and is
available free of charge [1].
[1]http://tech.knime.org/imagebee-analysing-imaging-data-from-the-honeybee-brain
[2] Martin Strauch, Julia Rein, C. Giovanni Galizia: Signal extraction from movies of honeybee brain
activity by convex analysis.
����167 Johannes Strauß
Spatial arrangement of auditory receptors in Tettigoniidae: a comparative tracing
study
Authors: Johannes Strauß 1 , Gerlind C. U. Lehmann 2 , Arne W. Lehmann, Reinhard
Lakes-Harlan 1
Affiliations: 1 Institute for Animal Physiology, AG Integrative Sensory Physiology,
Justus- Liebig-Universität Gießen; 2 Department of Biology, Behavioural
Physiology, Humboldt Universität zu Berlin
The auditory organ of Tettigoniidae is located in the proleg tibia and consists of
scolopidial sensilla organised in an array called crista acustica. This ear is a highly
ordered, tonotopic sensory system: the tonotopy of the receptors is the correlation
between the proximo-distal position of a receptor in the crista acustica on one hand,
and the distinct characteristic frequency of maximum sensitivity for an individual
receptor on the other hand. Detailed anatomical studies reported that the sensory
neurons and their dendrites in the distal crista acustica may occur in a double row
rather than in a strictly linear arrangement. With the physiological analysis of single
receptors and the established concept of tonotopy, the crista acustica became
depicted as a linear set of sensory elements. Here, the spatial arrangement of
receptor cell bodies and dendrites is shown by retrograde tracing with cobalt chloride
solution in six tettigoniid species. In these species, the distal receptor neurons are
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