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2. Behavioral Biology TALKS - Deutsche Zoologische Gesellschaft

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�79 Julius Braun A 703 / 15:00<br />

Morphological and chemical analyses of adhesive tarsus structures in insects<br />

Author: Julius Braun 1<br />

Affiliation: 1 Universität Tübingen<br />

Insects are able to adhere to even smooth surfaces during locomotion. The adhesive<br />

performance is provided by morphological features of the tarsal surface as well as<br />

the chemical properties of adhesive liquids. We conducted both morphological<br />

examinations of the tarsus structure and chemical analyses of tarsal liquids in several<br />

beetle species. We also measured the adhesive performance as a function of surface<br />

energy and roughness. To examine the chemical composition of the tarsal fluid we<br />

directly collected samples from the tarsal surfaces via both solid-phase microextraction<br />

and solvent extraction. Subsequent analyses of the secretion were<br />

conducted by means of hyphenation of gas-chromatography to mass-spectrometry<br />

(GC-MS). According to its high sensitivity in the microgram range, this method is wellsuited<br />

to characterize small sample amounts of the tarsal liquids.<br />

�80 Ute Eulitz A 703 / 15:15<br />

Coleoptera remains from a neolithic well 7200 years old<br />

Authors: Ute Eulitz 1 , Klaus-Dieter Klass 1<br />

Affiliation: 1 Senckenberg Naturhistorische Sammlungen Dresden, Museum für<br />

Tierkunde<br />

Wells from the Neolithic period of Central Europe (Linear Pottery Culture) are<br />

valuable archives for archaeological investigations and allow for a new perspective on<br />

this culture. Wood, ceramics, bones, pollen and insect remains have been conserved<br />

in sediments still humid today. Six neolithic wells (5500 - 5000 BC) were found in<br />

Saxony; that of Brodau near Leipzig (ca. 5200 BC; discovered in 2005) was<br />

investigated in this study. A total of 80 samples yielded 2387 arthropod fragments,<br />

mostly from Coleoptera (besides few others from Hymenoptera, Diptera and<br />

Araneae).<br />

Coleoptera remains are mostly isolated elytra, pronota and head capsules; legs,<br />

mouthparts or sternites are sparse. Conventional identification keys are not<br />

applicable to the fragments because they require consideration of several body parts.<br />

The fragments are usually well preserved including cuticular surface structures and<br />

occasionally colouration or hairs. We based species identification on (1) examination<br />

of fragments including details of the cuticular surface and (2) comparison with<br />

identified specimens in the institute's Coleoptera collection, using SEM and light<br />

microscopy. This revealed many previously unknown species-distinguishing<br />

characters, mainly on elytra. Yet, identification remains ambiguous in taxa including<br />

several very similar species. The beetle fragments were assigned to 20 families, 36<br />

genera and 27 species. More than 60 % are Aphodiinae (Scarabaeidae) especially<br />

Aphodius varians, A. granarius and Pleurophorus caesus. Four synanthropic species<br />

were detected: Tenebrio obscurus, Oryzaephilus surinamensis (oldest records for<br />

both), Sitophilus granarius and Tenebroides mauretanicus, probably all pests on<br />

cereals. Carabidae (ca. eight species), Ceutorhynchus (Curculionidae) and Platystethus<br />

(Staphylinidae) were also well represented.<br />

128

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