2. Behavioral Biology TALKS - Deutsche Zoologische Gesellschaft

In another approach, we examine developmental changes in the ALs neuropeptide
repertoire of adult beetles. To quantify those changes we use stable isotope-labeled
peptide analogues as an internal standard.
Supported by the DFG priority program SPP 1392 “Integrative Analysis of Olfaction”
(SCHA 678/13-1)
�104 Ricarda Scheiner A 701 / 14:45
Biogenic amine receptors, response thresholds and division of labor in honeybees
Authors: R. Scheiner 1 , T. Reim 1 , A. Toteva 1 , M. Thamm 1 , P. Chatterjee 1 , S. V. Yi 1
Affiliation: 1 University of Potsdam
Honey bees are well-known for their complex division of labor. But how does a bee
know which task she has to perform? A prominent current theory assumes that
differences in individual sensory response thresholds are the basis for division of
labor. Workers of a colony differ in their response thresholds for task-related stimuli
and therefore perform different tasks.
Experimental evidence for differences in sensory response thresholds is rare. We
demonstrate that both age-independent division of labor among pollen and nectar
foragers and age-dependent division of labor between hive bees and foragers is
accompanied by respective changes in sensory response thresholds for visual and
gustatory stimuli.
But which mechanisms regulate sensory responsiveness and division of labor? Our
data strongly suggest that division of labor is regulated via differential expression of
octopamine and tyramine receptors. Age-independent division of labor among
foragers correlates with differential methylation and splicing of the honeybee
tyramine receptor. Age-dependent division of labor, in contrast, seems to be
mediated by differential expression of the honey bee octopamine receptor. In
addition, we show that both tyramine and octopamine significantly affect sensory
responsiveness to gustatory and visual stimuli, locomotion and learning behavior.
These findings imply that tyramine and octopamine receptors have decisive
regulatory functions in honey bee division of labor.
�105 Marc Spehr A 701 / 15:00
Mitochondrial calcium mobilization is a key element in olfactory signaling
Authors: Marc Spehr 1 , Daniela Fluegge 1 , Lisa M. Moeller 1 , Annika Cichy 1 , Monika
Gorin 1 , Agnes Weth 1 , Sophie Veitinger 1 , Silvia Cainarca 1 , Stefan Lohmer 1 ,
Sabrina Corazza 1 , Eva M. Neuhaus 1 , Werner Baumgartner 1 , Jennifer Spehr 1
Affiliation: 1 Department of Chemosensation, Institute for Biology II, RWTH Aachen
University, Aachen
In olfactory sensory neurons (OSNs), cytosolic Ca2+ controls the gain and sensitivity
of olfactory signaling. Important components of the molecular machinery that
orchestrates OSN Ca2+ dynamics have been described, but key details are still
missing. Here, we demonstrate a critical physiological role of mitochondrial Ca2+
mobilization in mouse OSNs. Combining a novel mitochondrial Ca2+ imaging
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