PHYTO MEDIZIN Mitteilungen der Deutschen ... - Die DPG

Persistent Identifier: urn:nbn:de:0294-pm-2005-2-6
Gemeinschaft hinwiesen, zeigten die Pseudomonas-spezifischen Gele eine
deutliche Beeinflussung der relativen Abundanz von Pseudomonaden auch
drei Wochen nach der Inokulation. Die CLSM-Untersuchungen zeigten eine
recht heterogene Besiedlung der Wurzel für PRD16 und PRF116, wobei bei
der Wurzelinokulation insbesondere Bereiche der Wurzel besiedelt waren,
die ursprünglich getaucht wurden. Aber auch nach drei Wochen waren einige
dicht besiedelte Bereiche nachweisbar.
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DNA and RNA from bulk and rhizosphere soil. chapter 1.12: 159-169. In G. A.
Kowalchuk, F. J. de Bruijn, I. M. Head, Akkermans, A.D.L., and J. D. van Elsas,
editors.Molecular Microbial Ecology Manual, 2nd edition. Kluwer Academic Publishers,
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Götz, M., N.C.M. Gomes, A. Dratwinski, R. Costa, G. Berg, R. Peixoto, L. Mendonça-Hagler,
and K. Smalla. 2005. Survival of gfp-tagged antagonistic bacteria in
the rhizosphere of tomato plants and their effects on the indigenous bacterial community.
FEMS Microb. Ecol. Eingereicht.
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and K. Smalla. 2004. Monitoring GFP-tagged bacterial antagonists in the rhizosphere
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224
Biological control of apple scab by application of non – pathogenic microorganisms
to the plant phyllosphere of Malus domestica
Kürkcüoglu, Sophia 1 ; Piotrowski Markus 2 ; Gau Achim 1 1 Institut für Botanik, Universität
Hannover, Herrenhäuserstr. 2, 30419 Hannover, Germany; 2 Lehrstuhl für
Pflanzenphysiologie, Ruhr-Universität Bochum, D-44780 Bochum, Germany, Email:
kuerkcueoglu@aol.com
The biological control of apple scab by application of antagonistic microorganisms
to the plant phyllosphere is an alternative strategy to the frequent
treatment of plants by fungicides. As known from the plant rhizosphere,
where systemic accquired resistance can be induced by microorganisms, we
assume a similar way of interaction in the phyllosphere of plants. Analyses of
the protein composition of the apoplast by SDS-PAGE revealed dramatic
changes after in-oculation by the non-pathogenic Pseudomonas fluorescens
Bk3 to the phyllosphere of Malus domestica cv. Hosteiner Cox. Sequencing
of the induced proteins by ESI-Q-TOF mass spectrometry and homology
search identified these additional proteins as ß-1,3- glucanase, thaumatin-like
protein, chitinase and hevein-like protein. In contrast, we found a non-
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