Plant basal resistance - Universiteit Utrecht

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Chapter 4 DIMBOA-producing wild-type plants (BX1), or BX-deficient bx1 mutant plants. After 7, 14 and 21 days of growth, roots of BX1 and bx1 plants from 2 independent genetic lines were collected and analysed for colonisation by P. putida FBC004 and other (non-GFP expressing) culturable rhizobacteria. The first experiment was performed with soil that had been autoclaved once before to the start of the experiment (Figure 5A), presenting a relatively low competition environment for the introduced P. putida cells. Two-factor ANOVA of rhizosphere colonisation of plants from Line A revealed a statistically significant interaction between plant genotype (BX1 versus bx1) and bacterial cell type (P. putida versus others) at all three time-points (7 days: F 1,14 =9.151, p=0.009; 14 days: F 1,14 =43.432, p
101 Recruitment of beneficial bacteria by maize roots interaction between plant genotype and rhizobacterial cell type (Figure 5B). Together, these data indicate that BX exudation in non-autoclaved soil stimulates rhizosphere colonisation by P. putida of relatively young seedlings. This BX effect becomes variable by 14 days and is absent in 21 day-old plants. Figure 5: Rhizosphere colonisation of DIMBOA-producing (BX1) and DIMBOA-deficient (bx1) maize lines by green-fluorescent protein (GFP)-expressing P. putida and other culturable rhizobacteria in autoclaved (A) and non-autoclaved soil (B). P. putida cells were introduced into the soil prior to planting of maize seeds. Shown are average values (CFU g -1 root fresh weight ± SEM; n = 6-8), corresponding to P. putida or other rhizobacteria. Cells were enumerated after 7, 14 and 21 days of plant growth by plating root surface extractions onto solid agar medium. Probabilities indicate statistical differences between BX1 and bx1 plants within one line at each time-point (Holm-Šidák pairwise multiple comparisons) when two-factor ANOVA indicated a statistically significant main effect of BX genotype and/or a statistically significant interaction between BX genotype and bacterial cell type (α = 0.05).
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Plant basal resistance: genetics, b
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Most of the research described in t
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Promotor: Prof. dr. Ir. C.M.J. Piet
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10 CHAPTER 1 General Introduction A
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Chapter 1 (PRRs; Gomez-Gomez and Bo
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Chapter 1 by avirulent pathogens (R
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Chapter 1 Selective non-pathogenic
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Chapter 1 an endogenous plant signa
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Chapter 1 between defence signallin
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Chapter 1 rarely provides complete
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Chapter 1 genetic resources for Ara
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Chapter 1 metabolites are biosynthe
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Chapter 1 constitutively produced i
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Chapter 1 latest insights. This Cha
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ABSTRACT 33 Genetic dissection of b
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35 Genetic dissection of basal defe
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Natural variation in responsiveness
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Page 49 and 50: 49 Genetic dissection of basal defe
Page 57: 57 Genetic dissection of basal defe
Page 61 and 62: ABSTRACT 61 Role of benzoxazinoids
Page 63 and 64: 63 Role of benzoxazinoids in maize
Page 67 and 68: Chemical treatments and callose qua
Page 69 and 70: Aphid infestation stimulates apopla
Page 81 and 82: Table S1: Primers used for RT-qPCR
Page 86 and 87: 86 CHAPTER 4 Benzoxazinoids in root
Page 88 and 89: Chapter 4 INTRODUCTION Plants have
Page 90 and 91: Chapter 4 Our study presents eviden
Page 92 and 93: Chapter 4 Maize - P. putida FBC004
Page 94 and 95: Chapter 4 P. putida is tolerant to
Page 96 and 97: Chapter 4 Impact of DIMBOA on the P
Page 98 and 99: Chapter 4 PP5286 dut deoxyuridine 5
Page 102 and 103: Chapter 4 DISCUSSION The rhizospher
Page 104 and 105: Chapter 4 patterns, we considered t
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Page 108 and 109: 108 CHAPTER 5 General Discussion
Page 110 and 111: Chapter 5 QTL was caused by a polym
Page 112 and 113: Chapter 5 Figure 1: CYP81D11 regula
Page 114 and 115: Chapter 5 et al., 2009), and allele
Page 116 and 117: Chapter 5 defence against different
Page 118 and 119: Chapter 5 occurred at time-points l
Page 120 and 121: Chapter 5 rhizospheric signals that
Page 122 and 123: Chapter 5 glucosinolate profiles. 1
Page 124 and 125: References References Abramovitch R
Page 126 and 127: References QTL mapping and characte
Page 128 and 129: References Gianoli E, Niemeyer HM (
Page 130 and 131: References Kliebenstein DJ, Kroyman
Page 132 and 133: References Studies in Natural Produ
Page 134 and 135: References Geniostoma antherotrichu
Page 136 and 137: References Todesco M, Balasubramani
Page 138 and 139: References Walters DR, Paterson L,
Page 140 and 141: Summary Summary Basal resistance in
Page 142 and 143: Samenvatting Samenvatting Basale re
Page 144 and 145: Samenvatting inzichten opgeleverd i
Page 146 and 147: Acknowledgments Acknowledgements In
Page 148 and 149: Curriculum vitae Shakoor was born o
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