Plant basal resistance - Universiteit Utrecht

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Chapter 4 PP5286 dut deoxyuridine 5'-tri-phosphate nucleotido-hydrolase 98 Metabolism; Nucleotide Metabolism; Pyrimidine metabolism PP5296 gmk guanylate kinase Metabolism; Nucleotide Metabolism; Purine metabolism CHEMOTAXIS AND MOTILITY PP4888 Methyl-accepting chemotaxis sensory transducer PP4340 cheY two-component system, chemotaxis family, response regulator, CheY OTHER CELLULAR PROCESSES PP4315 PhzF family phenazine biosynthesis protein PP5000 hslV ATP-dependent protease peptidase subunit: Heat shock protein Environmental Information Processing; Signal Transduction; Twocomponent system. Cellular Processes; Cell Motility; Bacterial chemotaxis Cellular processes; adaptations to atypical conditions PP4874 rplI 50S ribosomal protein, L9 Genetic Information Processing; Translation; Ribosome PP4830 cobL Precorrin-6Y C5,15-methyltransferase subunit, CbiE Metabolism; Metabolism of Cofactors and Vitamins; Porphyrin and chlorophyll metabolism PP3446 ilvA-1 Threonine dehydratase Metabolism; Amino Acid Metabolism; Glycine, serine and threonine metabolism. Metabolism; Amino Acid Metabolism; Valine, leucine and isoleucine biosynthesis PP5110 ftsE cell division ATP-binding protein FtsE PP5226 Lppl family lipoprotein Environmental Information Processing; Membrane Transport; ABC transporters PP5398 ISPpu14 transposase Orf1 Transposition; DNA mediated. DNA binding. Transposase activity * DIMBOA-inducible genes with predicted functions in metabolism and transport of N-heteroaromatic compounds. See text for details.
DIMBOA induces positive chemotaxis by P. putida 99 Recruitment of beneficial bacteria by maize roots Based on the outcome of the transcriptome analysis, we examined the possibility that DIMBOA acts as a chemo-attractant for P. putida KT2440. A capillary-based assay was used to assess chemotactic behaviour to DIMBOA (Harwood et al., 1984). Significantly more cells (p=0.022; t-test) were attracted into capillaries containing 5 µg mL -1 DIMBOA compared to tubes with motility buffer alone (Figure 4). The average number of DIMBOA-attracted cells were statistically similar to the average number of cells that were attracted to the positive control tubes, containing 0.01% w/v casamino acids (Figure 4). Hence, P. putida KT2440 is attracted to DIMBOA in vitro. Figure 4: Chemotaxis of P. putida KT2440 towards DIMBOA. A capillary-based assay was used to assess chemotactic responses. Data represent average numbers of colony forming units (CFU ± SEM) from 1 μL glass capillaries containing motility buffer (control), 0.01% casamino acid (positive control), or 5 μg mL -1 DIMBOA. Cells were extracted from capillaries after 30 minutes of incubation and enumerated on solid medium. DIMBOA attracts P. putida to the rhizosphere Having established that DIMBOA induces chemotaxis-associated genes in P. putida KT2440, and that P. putida KT2440 is attracted to DIMBOA in vitro, we investigated whether these responses are biologically relevant in the maize rhizosphere. To this end, cells of GFP- expressing P. putida FBC004 were mixed into the soil prior to planting seeds of either
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Plant basal resistance: genetics, b
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Most of the research described in t
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Promotor: Prof. dr. Ir. C.M.J. Piet
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10 CHAPTER 1 General Introduction A
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Chapter 1 (PRRs; Gomez-Gomez and Bo
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Chapter 1 by avirulent pathogens (R
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Chapter 1 Selective non-pathogenic
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Chapter 1 an endogenous plant signa
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Chapter 1 between defence signallin
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Chapter 1 rarely provides complete
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Chapter 1 genetic resources for Ara
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Chapter 1 metabolites are biosynthe
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Chapter 1 constitutively produced i
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Chapter 1 latest insights. This Cha
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ABSTRACT 33 Genetic dissection of b
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35 Genetic dissection of basal defe
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Natural variation in responsiveness
Page 47 and 48: 47 Genetic dissection of basal defe
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Page 61 and 62: ABSTRACT 61 Role of benzoxazinoids
Page 63 and 64: 63 Role of benzoxazinoids in maize
Page 67 and 68: Chemical treatments and callose qua
Page 69 and 70: Aphid infestation stimulates apopla
Page 81 and 82: Table S1: Primers used for RT-qPCR
Page 86 and 87: 86 CHAPTER 4 Benzoxazinoids in root
Page 88 and 89: Chapter 4 INTRODUCTION Plants have
Page 90 and 91: Chapter 4 Our study presents eviden
Page 92 and 93: Chapter 4 Maize - P. putida FBC004
Page 94 and 95: Chapter 4 P. putida is tolerant to
Page 96 and 97: Chapter 4 Impact of DIMBOA on the P
Page 100 and 101: Chapter 4 DIMBOA-producing wild-typ
Page 102 and 103: Chapter 4 DISCUSSION The rhizospher
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Page 108 and 109: 108 CHAPTER 5 General Discussion
Page 110 and 111: Chapter 5 QTL was caused by a polym
Page 112 and 113: Chapter 5 Figure 1: CYP81D11 regula
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Page 116 and 117: Chapter 5 defence against different
Page 118 and 119: Chapter 5 occurred at time-points l
Page 120 and 121: Chapter 5 rhizospheric signals that
Page 122 and 123: Chapter 5 glucosinolate profiles. 1
Page 124 and 125: References References Abramovitch R
Page 126 and 127: References QTL mapping and characte
Page 128 and 129: References Gianoli E, Niemeyer HM (
Page 130 and 131: References Kliebenstein DJ, Kroyman
Page 132 and 133: References Studies in Natural Produ
Page 134 and 135: References Geniostoma antherotrichu
Page 136 and 137: References Todesco M, Balasubramani
Page 138 and 139: References Walters DR, Paterson L,
Page 140 and 141: Summary Summary Basal resistance in
Page 142 and 143: Samenvatting Samenvatting Basale re
Page 144 and 145: Samenvatting inzichten opgeleverd i
Page 146 and 147: Acknowledgments Acknowledgements In
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Curriculum vitae Shakoor was born o
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List of Publications Published arti
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Plant basal resistance - Universiteit Utrecht

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