PCR Detection of Microbial Pathogens PCR Detection of Microbial ...

Table 3
Conditions of PCR Assays for the Detection of Different Target Genes of STEC
PCR Amplicon
Primers Primer Sequences (5'-3') Target Gene Denaturation Annealing Extension (bp) References
hly A1 ggt gca gca gaa aaa gtt gta g EHEC-hlyA 94°C for 30s 57°C for 60s 72°C for 90s 1551 34
hlyA4 tct cgc ctg ata gtg ttt ggt a 30 cycles
SK1 ccc gaa ttc ggc aca agc ata agc eae 94°C for 30s 52°C for 60s 72°C for 60s 863 44
SK2 ccc gga tcc gtc tcg cca gta ttc g 30 cycles
AstA1 gcc atc aac aca gta tat ccg ast A 94°C for 30s 53°C for 60s 72°C for 50s 140 32
AstA2 gcg agt gac ggc ttt gta gt 30 cycles
wkat-B ctt cct gtt ctg att ctt ctg g kat P 94°C for 30s 56°C for 60s 72°C for 40s 2125 37
wkat-F aac tta ttt ctc gca tca tcc c 30 cycles
Esp A aaa cag cag gca ctt gaa cg esp P 94°C for 30s 56°C for 60s 72°C for 150s 1830 38
Esp B gga gtc gtc agt cag tag at 30 cycles
D1 cgt cag gag gat gtt cag etp D 94°C for 30s 52°C for 60 72°C for 70s 1062 39
D13R cga ctg cac ctg ttc ctg att a 30 cycles
356 gca gga tga ccc tgt aac gaa g ile X 94°C for 30s 52°C for 60s 72°C for 60s 640 41
595 ccg aag aaa aac cca gta aca g 30 cycles
col D1 gta aat ctg cct gtt cgt gga c col D 157 94°C for 30s 57°C for 60s 72°C for 60s 587 40
col D2 cct ttt tct ctt cgg tat gtt c 30 cycles
irp 2FP aag gat tcg ctg tta ccg gac irp 2 94°C for 60s 60°C for 60s 72°C for 60s 280 31
irp 2RP tcg tcg ggc agc gtt tct tct 30 cycles
fyu A FP gcg acg gga agc gat tta fyu A 94°C for 60s 57°C for 60s 72°C for 60s 780 31
fyu A RP cgc agt agg cac gat gtt gta 30 cycles
ANK 49 atg tta tcc tca tat aaa ata aac pas 94°C for 20s 50°C for 60s 72°C for 120s 1221 44
ANK 50 tta ata cga cag tgg aat atg 30 cycles
STEC PCR 173