PCR Detection of Microbial Pathogens PCR Detection of Microbial ...

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Standardization of PCR 53 Fig. 1. Graphical presentation of the FOOD-PCR project for validation and standardization of PCR for detection of important food-borne pathogens. 3. A Common Vision The experience gained through the FOOD-PCR project and activities of various international working groups suggest that a very basic aspect of PCR standardization is elaboration of a vision, or far sighted goals. As with any other major effort, the work should be based on a clear vision, a focused strategy,
54 Hoorfar and Cook Fig. 2. Practical steps when performing ring trials for validation of PCR. and a few basic principles shared by the organizations, industries, scientists and authorities involved. The vision adopted by the PCR working group (TAG3/WG6) of CEN is that diagnostic PCR will have the same status as conventional bacteriological culture techniques by the year 2010 (see Fig. 3). The strategy or long-term plan to achieve that vision is rapid publication of a few basic guidelines. The principles of the work will be based on noncommercial and international effort, including extensive multicenter ring trials, with the aim of providing end-users with nonexclusive protocols. The emphasis in the strategy is the speed and simplicity of the publications, thus avoiding protocols outdated by the amazing pace of technology development in the field of molecular diagnostics.
Page 1 and 2: TM Methods in Molecular Biology VOL
Page 3 and 4: 4 Sachse 2. Selection of Target Seq
Page 5 and 6: Table 1 Target Sequences Used in PC
Page 7 and 8: 8 Sachse Fig. 1. Structural organiz
Page 9 and 10: 10 Sachse proteins (66-68), invasio
Page 11 and 12: 12 Sachse Since there appears to be
Page 13 and 14: 14 Sachse In the context of identif
Page 15 and 16: 16 Sachse to partially compensate t
Page 17 and 18: 18 Sachse nostic potential of PCR.
Page 19 and 20: 20 Sachse product has to be confirm
Page 21 and 22: 22 Sachse 7. van Camp, G., Fierens,
Page 23 and 24: 24 Sachse 33. Rappelli, P., Are, R.
Page 25 and 26: 26 Sachse 60. Furrer, B., Candrian,
Page 27 and 28: 28 Sachse 89. Bloch, W. (1991) A bi
Page 29 and 30: 30 Sachse
Page 31 and 32: 32 Rådström et al. Fig. 1. Illust
Page 33 and 34: 34 Rådström et al. immunoglobulin
Page 35 and 36: 36 Rådström et al. and the captur
Page 37 and 38: Table 2 Comparison of the Performan
Page 39 and 40: 40 Rådström et al. explain these
Page 41 and 42: 42 Rådström et al. 5.1. Proteins
Page 43 and 44: 44 Rådström et al. neous. Consequ
Page 45 and 46: 46 Rådström et al. 11. Powell, H.
Page 47 and 48: 48 Rådström et al. 39. Katcher, H
Page 49 and 50: 50 Rådström et al. 73. Nagai, M.,
Page 51: 52 Hoorfar and Cook 2. FOOD-PCR: A
Page 55 and 56: 56 Hoorfar and Cook Fig. 4. The str
Page 57 and 58: Table 58 3 Hoorfar and Cook Test Co
Page 59 and 60: 60 Hoorfar and Cook The choosing an
Page 61 and 62: 62 Hoorfar and Cook 10. Demanding L
Page 63 and 64: 64 Hoorfar and Cook 15. Anon. (1995
Page 65 and 66: 66 Lübeck and Hoorfar ods for the
Page 67 and 68: 68 Lübeck and Hoorfar amplificatio
Page 69 and 70: 70 Lübeck and Hoorfar It may be ne
Page 71 and 72: 72 Lübeck and Hoorfar specificity
Page 73 and 74: 74 Lübeck and Hoorfar The latter i
Page 75 and 76: 76 Lübeck and Hoorfar A simple che
Page 77 and 78: 78 Lübeck and Hoorfar 13. Hanai, K
Page 79 and 80: 80 Lübeck and Hoorfar 43. Mitchell
Page 81 and 82: 82 Lübeck and Hoorfar 70. Lawrence
Page 83 and 84: 84 Lübeck and Hoorfar 98. Lantz, P
Page 85 and 86: 88 Frey Table 1 Presence of the Var
Page 87 and 88: 90 Frey 13. Lysis buffer: 100 mM Tr
Page 89 and 90: 92 Frey as template for PCR. In add
Page 91 and 92: 94 Frey strains, including all sero
Page 93 and 94: 96 Frey
Page 95 and 96: 98 Ewalt and Bricker (named for the
Page 97 and 98: 100 Ewalt and Bricker 4. Ethidium b
Page 99 and 100: 102 Ewalt and Bricker Primer Cockta
Page 101 and 102: 104 Ewalt and Bricker Fig. 2. Typic
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106 Ewalt and Bricker with betaine
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108 Ewalt and Bricker unused ethidi
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110 Englen et al. which campylobact
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112 Englen et al. 18. Dehydrated cu
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114 Englen et al. 3.1.3. Fecal Samp
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116 Englen et al. respectively), an
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118 Englen et al. Fig. 1. Identific
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120 Englen et al. 3. Goossens, H.,
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122 Englen et al.
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124 Sachse and Hotzel Table 1 Compa
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126 Sachse and Hotzel Table 2 Prime
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128 Sachse and Hotzel 7. Phenol: sa
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130 Sachse and Hotzel 4. Vortex mix
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132 Sachse and Hotzel Fig. 2. Speci
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134 Sachse and Hotzel In the latter
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136 Sachse and Hotzel 17. Longbotto
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138 Popoff spastic paralysis. The g
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Table 3 Primers for Toxin Gene Dete
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142 Popoff 18. Agarose gel loading
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144 Popoff Taq reaction buffer 1X,
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146 Popoff almost all C. perfringen
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148 Popoff 2. Add to each PCR tube
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150 Popoff 7. Hielm, S., Hyyttia, E
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152 Popoff 35. Wieckowski, E., Bill
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154 Berri et al. 2. Materials 1. C.
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156 Berri et al. 3.3.2. Vaginal Swa
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158 Berri et al. Fig. 1. Trans-PCR.
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160 Berri et al. Fig. 3. Sensitivit
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162 Berri et al.
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164 Gallien The first description o
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166 Gallien
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168 Gallien 2. Materials 2.1. Bacte
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170 Gallien 10. Ethidium bromide: 1
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Table 2 Components (in µL) of 25-
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Table 4 Components (in µL) of 25-
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176 Gallien 3.3. Specific Isolation
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Table 6 PCR Conditions for Subtypin
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180 Gallien Fig. 3. Photographic im
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182 Gallien 3. Boyce, T. G., Swerdl
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184 Gallien enteropathogenic E. col
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186 O'Connor This chapter will desc
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188 O'Connor 2.2. PCR of Enriched F
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190 O'Connor 4. Notes 1. In enrichi
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192 O'Connor 4. O’Sullivan, N., F
Page 191 and 192:
194 Lester and LeFebvre titers (4).
Page 193 and 194:
196 Lester and LeFebvre 3.1.2. Samp
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Fig. 1. Sensitivity of the PCR assa
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200 Lester and LeFebvre 5. Swart, K
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202 Skuce et al. Table 1 Significan
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204 Skuce et al. commercial kits. E
Page 203 and 204:
206 Skuce et al. Fig. 1. Schematic
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208 Skuce et al. 5. Disposable ster
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210 Skuce et al. 2.5.2. Sequence An
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212 Skuce et al. 2. Carefully trans
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Table 4 Recommended PCR Amplificati
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216 Skuce et al. Fig. 3. PCR produc
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218 Skuce et al. Fig. 5. Spoligotyp
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220 Skuce et al. 14. Aranaz, A., Li
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222 Skuce et al.
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224 Khan Simultaneous detection of
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226 Khan 10. Incubate for 20 min at
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228 Khan 5. Periodically, multiplex
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230 Khan
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232 Hotzel et al. tis (2). Particul
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234 Hotzel et al. 8. Sodium dodecyl
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236 Hotzel et al. 3. Methods 3.1. D
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238 Hotzel et al. 3.1.5. Semen (see
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240 Hotzel et al. Fig. 1 Detection
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242 Hotzel et al. 4. Notes 1. The u
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244 Hotzel et al. in Mycoplasma Pro
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246 Hotzel et al.
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248 Kobisch and Frey PCR assay to d
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250 Kobisch and Frey Table 1 Oligon
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252 Kobisch and Frey 3.2.1. Prepara
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254 Kobisch and Frey First round of
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256 Kobisch and Frey References 1.
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258 Christensen et al. thrombotic m
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260 Christensen et al. Table 1 Spec
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Table 2 Oligonucleotide Primers for
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264 Christensen et al. 3.2. Extract
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Table 3 PCR Protocols for the Patho
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268 Christensen et al. 2. Apply fra
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270 Christensen et al. 7. To reduce
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272 Christensen et al. 24. Fisher,
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274 Christensen et al. 54. Hunt, M.
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276 Malorny and Helmuth valent meta
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278 Malorny and Helmuth 12. Apparat
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280 Malorny and Helmuth 3.3. Amplif
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282 Malorny and Helmuth Table 2 Vol
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284 Malorny and Helmuth Table 4 PCR
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286 Malorny and Helmuth 3. Wallace,
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288 Malorny and Helmuth
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290 Wastling and Mattsson is an imp
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292 Wastling and Mattsson 2. Remove
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294 Wastling and Mattsson Fig. 1. B
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296 Wastling and Mattsson time PCR
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298 Wastling and Mattsson
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300 Pozio and La Rosa Table 1 Princ
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302 Pozio and La Rosa regions; spot
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304 Pozio and La Rosa 3. Proteinase
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306 Pozio and La Rosa Table 3 PCR-R
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308 Pozio and La Rosa 2. Pepsin sho
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310 Pozio and La Rosa
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312 Knutsson and Rådström The ref
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314 Knutsson and Rådström Fig. 1.
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316 Knutsson and Rådström 2.4. El
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318 Knutsson and Rådström the swa
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320 Knutsson and Rådström Fig. 3.
Page 319 and 320:
322 Knutsson and Rådström 4. Alek
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324 Knutsson and Rådström 34. Hee
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