PCR Detection of Microbial Pathogens PCR Detection of Microbial ...

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Avian Mycoplasma PCR 229 12. Zhao, S., and Yamamoto, R. (1993) Amplification of Mycoplasma iowae using polymerase chain reaction. Avian Dis. 37, 212–217. 13. Zhao, S., and Yamamoto, R. (1993) Detection of Mycoplasma synoviae by polymerase chain reaction. Avian Pathol. 22, 533–542. 14. Zhao, S., and Yamamoto, R. (1993) Detection of Mycoplasma meleagridis by polymerase chain reaction. Vet. Microbiol. 36, 91–97. 1993. 15. Bradbury J. M., and McClenaghan M. (1982) Detection of mixed mycoplasma species. J. Clin. Microbiol. 16, 314–318. 16. Rott M., Pfützner, H., Gigas, H., and Rott, G.( 1989) Diagnostic experiences in the routine restrained inspection of turkey stock for mycoplasma infections. Arch. Exper. Vet. Med. 43, 743–746. 17. Sahu, S. P., and Olson, N. O. (1981) Characterization of an isolate of Mycoplasma WVU 907 which possesses common antigens to Mycoplasma gallisepticum. Avian Dis. 25, 943–953. 18. Way, J. S., Josephson, K. L., Pillai, S. D., Abbaszadegan, M., Gerba, C. P, and Pepper, I. L. (1993) Specific detection of Salmonella spp. by multiplex polymerase chain reaction. Appl. Environ. Microbiol. 59, 1473–1479. 19. Kulski, J. K., Khinsoe, C., Pryce, T., and Christiansen, K. (1995) Use of multiplex PCR to detect and identify Mycobacterium avium and M. intercellulare in blood culture fluids of AIDS patients. J. Clin Microbiol. 33, 668–74. 20. Lawrence, L. M., and Gilmour, A. (1994) Incidence of Listeria spp. And Listeria monocytogenes in a poultry-processing environment and in poultry products and their confirmation by multiplex PCR. Appl. Environ. Microbiol. 60, 4600–4604. 21. Karlsen, F., Kalantari, M., Jenkins, A., et al. (1996) Use of multiplex PCR primer sets for optimal detection of human papillomavirus. J. Clin. Microbiol. 34, 2095–2100. 22. Wang, X., and Khan, M. I. (1999) A multiplex PCR for Massachusetts and Arkansas serotypes of infectious bronchitis virus. Mol. Cell. Probes 13, 1–7. 23. Chamberlain, J. S., Gibbs, R. A., Ranier, J. E., Nguyen, P. N., and Caskey, C. T. (1981) Deletion screening of the duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acid Res. 16, 11141–56. 24. Wang, Han., Fadl, A. A, and Khan, M. I. (1996) Multiplex PCR for avian pathogenic mycoplasmas. Mol. Cell Probes 11, 211–216. 25. Sambrook, J., Fritsch, E. T., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. pp. #1.25, 1.85, 6.3, 9.47, A.1, E.3, E.5. CSH Laboratory Press, Cold Spring Harbor, NY, USA. 26. Frey, M. L., Hanson, R. P., and Anderson, D. P. (1968) A medium for the isolation of avian mycoplasmas. Am. J. Vet. Res. 29, 2163–2171.
230 Khan
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TM Methods in Molecular Biology VOL
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4 Sachse 2. Selection of Target Seq
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Table 1 Target Sequences Used in PC
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8 Sachse Fig. 1. Structural organiz
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10 Sachse proteins (66-68), invasio
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12 Sachse Since there appears to be
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14 Sachse In the context of identif
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16 Sachse to partially compensate t
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18 Sachse nostic potential of PCR.
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20 Sachse product has to be confirm
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22 Sachse 7. van Camp, G., Fierens,
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24 Sachse 33. Rappelli, P., Are, R.
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26 Sachse 60. Furrer, B., Candrian,
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28 Sachse 89. Bloch, W. (1991) A bi
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30 Sachse
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32 Rådström et al. Fig. 1. Illust
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34 Rådström et al. immunoglobulin
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36 Rådström et al. and the captur
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Table 2 Comparison of the Performan
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40 Rådström et al. explain these
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42 Rådström et al. 5.1. Proteins
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44 Rådström et al. neous. Consequ
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46 Rådström et al. 11. Powell, H.
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48 Rådström et al. 39. Katcher, H
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50 Rådström et al. 73. Nagai, M.,
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52 Hoorfar and Cook 2. FOOD-PCR: A
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54 Hoorfar and Cook Fig. 2. Practic
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56 Hoorfar and Cook Fig. 4. The str
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Table 58 3 Hoorfar and Cook Test Co
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60 Hoorfar and Cook The choosing an
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62 Hoorfar and Cook 10. Demanding L
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64 Hoorfar and Cook 15. Anon. (1995
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66 Lübeck and Hoorfar ods for the
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68 Lübeck and Hoorfar amplificatio
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70 Lübeck and Hoorfar It may be ne
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72 Lübeck and Hoorfar specificity
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74 Lübeck and Hoorfar The latter i
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76 Lübeck and Hoorfar A simple che
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78 Lübeck and Hoorfar 13. Hanai, K
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80 Lübeck and Hoorfar 43. Mitchell
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82 Lübeck and Hoorfar 70. Lawrence
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84 Lübeck and Hoorfar 98. Lantz, P
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88 Frey Table 1 Presence of the Var
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90 Frey 13. Lysis buffer: 100 mM Tr
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92 Frey as template for PCR. In add
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94 Frey strains, including all sero
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96 Frey
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98 Ewalt and Bricker (named for the
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100 Ewalt and Bricker 4. Ethidium b
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102 Ewalt and Bricker Primer Cockta
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104 Ewalt and Bricker Fig. 2. Typic
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106 Ewalt and Bricker with betaine
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108 Ewalt and Bricker unused ethidi
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110 Englen et al. which campylobact
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112 Englen et al. 18. Dehydrated cu
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114 Englen et al. 3.1.3. Fecal Samp
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116 Englen et al. respectively), an
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118 Englen et al. Fig. 1. Identific
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120 Englen et al. 3. Goossens, H.,
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122 Englen et al.
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124 Sachse and Hotzel Table 1 Compa
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126 Sachse and Hotzel Table 2 Prime
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128 Sachse and Hotzel 7. Phenol: sa
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130 Sachse and Hotzel 4. Vortex mix
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132 Sachse and Hotzel Fig. 2. Speci
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134 Sachse and Hotzel In the latter
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136 Sachse and Hotzel 17. Longbotto
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138 Popoff spastic paralysis. The g
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Table 3 Primers for Toxin Gene Dete
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142 Popoff 18. Agarose gel loading
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144 Popoff Taq reaction buffer 1X,
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146 Popoff almost all C. perfringen
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148 Popoff 2. Add to each PCR tube
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150 Popoff 7. Hielm, S., Hyyttia, E
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152 Popoff 35. Wieckowski, E., Bill
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154 Berri et al. 2. Materials 1. C.
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156 Berri et al. 3.3.2. Vaginal Swa
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158 Berri et al. Fig. 1. Trans-PCR.
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160 Berri et al. Fig. 3. Sensitivit
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162 Berri et al.
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164 Gallien The first description o
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166 Gallien
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168 Gallien 2. Materials 2.1. Bacte
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170 Gallien 10. Ethidium bromide: 1
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Table 2 Components (in µL) of 25-
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Table 4 Components (in µL) of 25-
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176 Gallien 3.3. Specific Isolation
Page 175 and 176: Table 6 PCR Conditions for Subtypin
Page 177 and 178: 180 Gallien Fig. 3. Photographic im
Page 179 and 180: 182 Gallien 3. Boyce, T. G., Swerdl
Page 181 and 182: 184 Gallien enteropathogenic E. col
Page 183 and 184: 186 O'Connor This chapter will desc
Page 185 and 186: 188 O'Connor 2.2. PCR of Enriched F
Page 187 and 188: 190 O'Connor 4. Notes 1. In enrichi
Page 189 and 190: 192 O'Connor 4. O’Sullivan, N., F
Page 191 and 192: 194 Lester and LeFebvre titers (4).
Page 193 and 194: 196 Lester and LeFebvre 3.1.2. Samp
Page 195 and 196: Fig. 1. Sensitivity of the PCR assa
Page 197 and 198: 200 Lester and LeFebvre 5. Swart, K
Page 199 and 200: 202 Skuce et al. Table 1 Significan
Page 201 and 202: 204 Skuce et al. commercial kits. E
Page 203 and 204: 206 Skuce et al. Fig. 1. Schematic
Page 205 and 206: 208 Skuce et al. 5. Disposable ster
Page 207 and 208: 210 Skuce et al. 2.5.2. Sequence An
Page 209 and 210: 212 Skuce et al. 2. Carefully trans
Page 211 and 212: Table 4 Recommended PCR Amplificati
Page 213 and 214: 216 Skuce et al. Fig. 3. PCR produc
Page 215 and 216: 218 Skuce et al. Fig. 5. Spoligotyp
Page 217 and 218: 220 Skuce et al. 14. Aranaz, A., Li
Page 219 and 220: 222 Skuce et al.
Page 221 and 222: 224 Khan Simultaneous detection of
Page 223 and 224: 226 Khan 10. Incubate for 20 min at
Page 225: 228 Khan 5. Periodically, multiplex
Page 229 and 230: 232 Hotzel et al. tis (2). Particul
Page 231 and 232: 234 Hotzel et al. 8. Sodium dodecyl
Page 233 and 234: 236 Hotzel et al. 3. Methods 3.1. D
Page 235 and 236: 238 Hotzel et al. 3.1.5. Semen (see
Page 237 and 238: 240 Hotzel et al. Fig. 1 Detection
Page 239 and 240: 242 Hotzel et al. 4. Notes 1. The u
Page 241 and 242: 244 Hotzel et al. in Mycoplasma Pro
Page 243 and 244: 246 Hotzel et al.
Page 245 and 246: 248 Kobisch and Frey PCR assay to d
Page 247 and 248: 250 Kobisch and Frey Table 1 Oligon
Page 249 and 250: 252 Kobisch and Frey 3.2.1. Prepara
Page 251 and 252: 254 Kobisch and Frey First round of
Page 253 and 254: 256 Kobisch and Frey References 1.
Page 255 and 256: 258 Christensen et al. thrombotic m
Page 257 and 258: 260 Christensen et al. Table 1 Spec
Page 259 and 260: Table 2 Oligonucleotide Primers for
Page 261 and 262: 264 Christensen et al. 3.2. Extract
Page 263 and 264: Table 3 PCR Protocols for the Patho
Page 265 and 266: 268 Christensen et al. 2. Apply fra
Page 267 and 268: 270 Christensen et al. 7. To reduce
Page 269 and 270: 272 Christensen et al. 24. Fisher,
Page 271 and 272: 274 Christensen et al. 54. Hunt, M.
Page 273 and 274: 276 Malorny and Helmuth valent meta
Page 275 and 276: 278 Malorny and Helmuth 12. Apparat
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280 Malorny and Helmuth 3.3. Amplif
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282 Malorny and Helmuth Table 2 Vol
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284 Malorny and Helmuth Table 4 PCR
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286 Malorny and Helmuth 3. Wallace,
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288 Malorny and Helmuth
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290 Wastling and Mattsson is an imp
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292 Wastling and Mattsson 2. Remove
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294 Wastling and Mattsson Fig. 1. B
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296 Wastling and Mattsson time PCR
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298 Wastling and Mattsson
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300 Pozio and La Rosa Table 1 Princ
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302 Pozio and La Rosa regions; spot
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304 Pozio and La Rosa 3. Proteinase
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306 Pozio and La Rosa Table 3 PCR-R
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308 Pozio and La Rosa 2. Pepsin sho
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310 Pozio and La Rosa
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312 Knutsson and Rådström The ref
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314 Knutsson and Rådström Fig. 1.
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316 Knutsson and Rådström 2.4. El
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318 Knutsson and Rådström the swa
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320 Knutsson and Rådström Fig. 3.
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322 Knutsson and Rådström 4. Alek
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324 Knutsson and Rådström 34. Hee
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