PCR Detection of Microbial Pathogens PCR Detection of Microbial ...

PCR Technology 77
automation event into PCR technology. The real-time mode of amplification
has basically abolished the need to open PCR tubes following amplification,
which is the main source of carryover.
In addition, application of solution hybridization probes in combination with
fluorescence dyes can increase diagnostic specificity and sensitivity of PCR
testing, making the use of nested PCR or double PCR unnecessary.
Finally, automation has increased the throughput capacity of PCR laboratories
substantially, providing a real opportunity for cost-effective testing of
large number of samples, with minimum requirements for skilled labor and
large dedicated work spaces. The major automation effort of the future will
be directed at pre-PCR sample treatment.
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