PCR Detection of Microbial Pathogens PCR Detection of Microbial ...

Standardization of PCR 59
Fig. 5. The proper sequence of events in preparation of PCR standards.
8. Sequence of Events
The occurrence of an incorrect sequence of events is seen with some of the
traditional culture techniques. When many workers begin to face the challenge
of quality control requirements it is realized that we actually have to go
several steps back and begin with proper validations (see Fig. 5). The writing
of standards must be based on validation studies and experience gained
through ring trials. Harmonization and validation are the prerequisites of standardization,
in that sequence, and the writing of standards must be based on
successful validation studies and laboratory experience gained through ring
trials. Implementation of quality assurance programs is often substantially
facilitated if relevant standards are available.
9. Development and Validation of Standard PCR-Based Methods
to Detect Food-Borne Pathogens
We propose that development and validation of a standard PCR-based
method to detect a food-borne pathogen should take place in three phases.