Review of the Food-borne Zoonoses Research ... - ARCHIVE: Defra

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if the vaccines currently available are effective against other S. Enteritidis phage types prevalent in the rest of Europe. The work will contribute significantly to the evidence base and should result in improved methods of control. The project is intended to assist the poultry layer industry to reduce levels of salmonella and it is important that the industry remains involved. 54
Project code: OZ0313 Project title: Non-specific and innate resistance to Salmonella infection in chickens and pigs Start date (dd/mm/yy): 01/04/99 End date (dd/mm/yy): 30/06/02 £689,400 Total cost: Affiliation: Institute for Animal Health Abstract of research We have studied the biological and cellular basis of the difference in in vivo killing of Salmonella strain by chicken lines which are either resistant or susceptible to systemic salmonellosis. Resistance correlated with killing by macrophages cultured in vitro from the blood of the respective chicken lines. This also correlated with the extent to which antibacterial oxidative radicals were produced in response to in vitro infection. This is a useful biological marker of this important genetic trait. Infection of epithelial cells by different Salmonella serovars provoked different responses. S. Typhimurium induced high levels of the pro-inflammatory chemokines IL-1 and IL-6 whereas S. Gallinarum was able to suppress their production. The suppressive capabilities of Salmonella strains was also demonstrated by virulent strains of S. Typhimurium in murine macrophages which were able to down-regulate the production of nitric oxide through the NF kappa B pathway. The induction of innate responses by live vaccines and their protective ability has been demonstrated in gnotobiotic pigs which has been found to be mediated by the induction of IL-8 induced neutrophils into the villus. The stimulation of the innate response by live vaccines is a useful attribute. We have also found the neutrophils have the potential to act as antigen presenting cells through the expression of the B17 co stimulatory molecule. Review Summary The project was appropriate at the time it was commissioned. It was carried out to a high standard and produced interesting results. It has considerably advanced the understanding of some of the fundamental mechanisms for establishment and clearance of salmonella infections in chickens and pigs. The goals of the research, namely 1) to determine the cellular basis for the difference in susceptibility to systemic salmonellosis in inbred chicken lines, and 2) to determine the basis for the stimulation of non-specific immunity in the pig intestine following intestinal colonisation by an avirulent rough Salmonella Infantis strain, have been achieved. The pig work demonstrated effective activation of innate immune response by one serotype (S. Infantis) to protect from disease induced by another serotype (S. Typhimurium). However, this protection did not reduce the colonisation of the intestine so, as in the chicken work, it is more relevant to control of animal disease than to the zoonoses. 55
Page 2 and 3:
Contents Section A - Introduction..
Page 4 and 5: Review of the Food-borne Zoonoses (
Page 6 and 7: Plans for taking forward these prio
Page 8 and 9: Policy Objectives The key policy ob
Page 10 and 11: Figure 2. Laboratory reports of Sal
Page 12 and 13: Figure 4. Laboratory reports of Cam
Page 14 and 15: 6. Aims of the FBZ research review
Page 16 and 17: Section B - Review Summaries 16
Page 18 and 19: epidemiology of Salmonella and anti
Page 20 and 21: There is a need to objectively asse
Page 22 and 23: 2. Campylobacter review summary 2.1
Page 24 and 25: for this organism. Research within
Page 26 and 27: 3. E. coli review summary 3.1 Succe
Page 28 and 29: 4. Other zoonotic pathogens of inte
Page 30 and 31: Annex 1 - Projects Reviewed 30
Page 32 and 33: OZ0311 Conventional versus capillar
Page 34 and 35: OZ0402 OZ0404 OZ0405 OZ0407 OZ0604
Page 36 and 37: OZ0704 OZ0705 OZ0706 OZ0707 Quantif
Page 38 and 39: Annex 2 - Project Abstracts and Pro
Page 40 and 41: To test the suitability of the AFLP
Page 42 and 43: Project code: OZ0324 Project title:
Page 46 and 47: Review Summary The knowledge that t
Page 48 and 49: Review Summary The project was felt
Page 52 and 53: It is clear that the EC legislative
Page 56 and 57: Four published papers have come out
Page 58 and 59: Review Summary This epidemiological
Page 60 and 61: Project code: OZO316 Project title:
Page 64 and 65: agricultural industry/public health
Page 66 and 67: testing laboratories. In addition,
Page 68 and 69: animal pathogen but is a human path
Page 70 and 71: esearch has also substantially impr
Page 72 and 73: to elucidate the antigenic repertoi
Page 74 and 75: and have contributed to our underst
Page 76 and 77: Fundamental issues concerning the f
Page 78 and 79: Review summary Project OZ0606 focus
Page 80 and 81: Abstract of research OZ0608 The ado
Page 82 and 83: thinning process for molecular trac
Page 84 and 85: Project code: LK0665 Project title:
Page 86 and 87: Review summary While poultry meat i
Page 88 and 89: Review summary A key aim of this pr
Page 90 and 91: Review summary This project aimed t
Page 96 and 97: Project code: OZ0138C Project title
Page 102 and 103: Abstract of research Projects OZ070
Page 104 and 105:
produced were both relevant and imp
Page 106 and 107:
List of External Reviewers. Mr. Pau
Page 108 and 109:
Review of Defra’s FBZ Research Pr
Page 110 and 111:
Review of Defra’s FBZ Research Pr
Page 112 and 113:
Annex 5 - Review Timetable 112
Page 114 and 115:
11.45 - 12.00 Bovine and Porcine Fo
Page 116:
Campylobacter Other 11.50 - 12.05 P
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