Review of the Food-borne Zoonoses Research ... - ARCHIVE: Defra

More documents

Recommendations

Info

Review summary While poultry meat is generally accepted as the primary source of infection for cases of human campylobacteriosis, there is also evidence to suggest that a significant proportion of these infections originate from alternative sources. The primary aim of this project was to determine carriage of Campylobacter spp. by the UK dog population, and any risk this may cause to human health. Results to-date indicate that dogs are not an important source of C. jejuni or C. coli, the common cause of infection in humans. C. upsaliensis was the most common campylobacter species detected in dogs. While this organism is zoonotic, it is recovered much less frequently from human campylobacter infections. 86
Project code: VTEC O157 Research Projects OZ0703 Project title: Plant antibody delivery of passive immunisation against E. coli O157:H7: a novel means of control in the animal Start date (dd/mm/yy): 01/09/1999 End date (dd/mm/yy): 01/06/2003 £354,108 Total cost: Affiliation: Veterinary Laboratories Agency Sub-contractor(s): ADAS Consulting Abstract of research Biology Department, University of Leicester Intimin, TIR and EspA proteins are expressed by Enterohaemorrhagic E. coli O157:H7. EspA proteins are part of the type three secretion system needle complex that delivers TIR to the host epithelial cell whilst surface arrayed intimin docks the bacterium to the translocated TIR. This process leads to intimate attachment resulting in attaching and effacing lesions which are essential for colonisation and persistence in ruminants. Recombinant forms of these effector proteins from E. coli O157:H7 were produced and used to elicit immune responses in rabbits and immune phage-display antibody libraries were produced. Screening of these immune libraries by phage-antibody panning and colony filter screening produced a panel of antibodies with specificity for EspA or intimin. Antibodies recognising different C-terminal epitopes on intimin bound specifically to the gamma intimin of O157:H7 and not to other classes of intimin whereas antibodies raised against EspA recognised EspA analogues from other serotypes also. Anti-intimin antibodies were also produced as fusion proteins coupled to the reporter molecule alkaline phosphatase, allowing the one-step detection of γ-intimin. The isolated recombinant monoclonal antibodies were functional in a range of assay formats including ELISA, Western blot and dot blots demonstrating diagnostic potential. We assessed whether these antibody constructs could be used to disrupt the intimate association of E. coli O157:H7 with the host cell but neither anti-γ intimin or anti-EspA antibodies did so although the anti-EspA antibodies significantly reduced the extent of E. coli O157:H7-induced host cell actin rearrangement. In tests with traditionally made antibodies, both monoclonal and polyclonal antibodies completely blocked adherence and cytoskeletal changes within the host cell. Both polyclonal and monoclonal antibodies could be used to label E. coli O157 EspA filaments and these immunoreagents did not inhibit the formation of such filaments. This is the first report of monoclonal antibodies to EspA capable of disrupting the TTSS function of E. coli O157:H7. 87
Page 2 and 3:
Contents Section A - Introduction..
Page 4 and 5:
Review of the Food-borne Zoonoses (
Page 6 and 7:
Plans for taking forward these prio
Page 8 and 9:
Policy Objectives The key policy ob
Page 10 and 11:
Figure 2. Laboratory reports of Sal
Page 12 and 13:
Figure 4. Laboratory reports of Cam
Page 14 and 15:
6. Aims of the FBZ research review
Page 16 and 17:
Section B - Review Summaries 16
Page 18 and 19:
epidemiology of Salmonella and anti
Page 20 and 21:
There is a need to objectively asse
Page 22 and 23:
2. Campylobacter review summary 2.1
Page 24 and 25:
for this organism. Research within
Page 26 and 27:
3. E. coli review summary 3.1 Succe
Page 28 and 29:
4. Other zoonotic pathogens of inte
Page 30 and 31:
Annex 1 - Projects Reviewed 30
Page 32 and 33:
OZ0311 Conventional versus capillar
Page 34 and 35:
OZ0402 OZ0404 OZ0405 OZ0407 OZ0604
Page 36 and 37: OZ0704 OZ0705 OZ0706 OZ0707 Quantif
Page 38 and 39: Annex 2 - Project Abstracts and Pro
Page 40 and 41: To test the suitability of the AFLP
Page 42 and 43: Project code: OZ0324 Project title:
Page 46 and 47: Review Summary The knowledge that t
Page 48 and 49: Review Summary The project was felt
Page 52 and 53: It is clear that the EC legislative
Page 54 and 55: if the vaccines currently available
Page 56 and 57: Four published papers have come out
Page 58 and 59: Review Summary This epidemiological
Page 60 and 61: Project code: OZO316 Project title:
Page 64 and 65: agricultural industry/public health
Page 66 and 67: testing laboratories. In addition,
Page 68 and 69: animal pathogen but is a human path
Page 70 and 71: esearch has also substantially impr
Page 72 and 73: to elucidate the antigenic repertoi
Page 74 and 75: and have contributed to our underst
Page 76 and 77: Fundamental issues concerning the f
Page 78 and 79: Review summary Project OZ0606 focus
Page 80 and 81: Abstract of research OZ0608 The ado
Page 82 and 83: thinning process for molecular trac
Page 84 and 85: Project code: LK0665 Project title:
Page 88 and 89: Review summary A key aim of this pr
Page 90 and 91: Review summary This project aimed t
Page 96 and 97: Project code: OZ0138C Project title
Page 102 and 103: Abstract of research Projects OZ070
Page 104 and 105: produced were both relevant and imp
Page 106 and 107: List of External Reviewers. Mr. Pau
Page 108 and 109: Review of Defra’s FBZ Research Pr
Page 110 and 111: Review of Defra’s FBZ Research Pr
Page 112 and 113: Annex 5 - Review Timetable 112
Page 114 and 115: 11.45 - 12.00 Bovine and Porcine Fo
Page 116: Campylobacter Other 11.50 - 12.05 P
show all

Review of the Food-borne Zoonoses Research ... - ARCHIVE: Defra

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?