Fundamental Organic Chemistry
Fundamental Organic Chemistry
Fundamental Organic Chemistry
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Fig.(7):Thin-layer Chromatography.<br />
The R f Value:<br />
The distance that the spot of a particular compound moves up the plate<br />
relative to the distance moved by the solvent front is called the retention factor,<br />
distance traveled by the compound<br />
R f = --------------------------------------------<br />
distance traveled by the solvent<br />
Equipment for TLC:<br />
TLC sheets and plates. Commercial TLC sheets are coated with silica gel<br />
(SiO 2 ) or alumina (Al 2 O 3 ). Choose the type that gives the best separation for your<br />
particular mixture.<br />
If commercial TLC sheets are unavailable, plates can be made from<br />
microscope slides and a slurry of 1g aluminum oxide G or silica gel G and 2mL<br />
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