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guide to thin section microscopy - Mineralogical Society of America

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Guide <strong>to</strong> Thin Section Microscopy<br />

Double refraction<br />

are "filtered out", while red and blue hues dominate. The range Γ = 500 ↔ 1500 nm is<br />

characterized by relatively intense colours due <strong>to</strong> elimination or reduction <strong>of</strong> one or two<br />

relatively narrow spectral intervals for each Γ value. For high retardation values (Γ > 1500<br />

nm) an increasing number <strong>of</strong> spectral domains distributed over the full wavelength spectrum<br />

is subtracted which produces increasingly pale colour <strong>to</strong>nes and, at very high Γ, an<br />

interference colour that is perceived as white. As can be derived from Fig. 4-27, at very high<br />

retardation the interference colour spectrum consists <strong>of</strong> a narrow-spaced and evenly<br />

distributed arrangement <strong>of</strong> domains <strong>of</strong> constructive and destructive interference, such that<br />

none <strong>of</strong> the major colour bands is cut out completely.<br />

Thus, the interference colour spectrum starts with black (Γ = 0) and progresses through grey,<br />

white, yellow, and orange <strong>to</strong> a succession <strong>of</strong> intense colours <strong>of</strong> red → blue → green → yellow<br />

→ orange → red, which repeats itself with increasing retardation, while getting more and<br />

more pale (Fig. 4-29; Michel-Lévy colour chart). The colour sequence is subdivided in<strong>to</strong><br />

colour orders using the distinctive purplish reds (in steps <strong>of</strong> 551 nm). From the 4 th order<br />

upwards, the interference colours are dominated by alternating greenish and reddish hues.<br />

With increasing retardation these fade more and more and eventually approach white (Fig. 4-<br />

33). This is referred <strong>to</strong> as high-order white (in contrast <strong>to</strong> first-order white).<br />

Raith, Raase & Reinhardt – February 2012<br />

The first graphical presentation between retardation, crystal thickness and birefringence [Γ =<br />

d*(n z '–n x ')] was published by Michel-Lévy (1888, Tableau des biréfringences in "Les<br />

Minéraux des Roches", Paris). This interference colour chart which shows just over four<br />

colour orders is used <strong>to</strong> this day as a useful standard <strong>to</strong>ol for mineral determination at the<br />

microscope. Improvements in printing technique over time ensured that modern colour charts<br />

are a reasonably close reproduction <strong>of</strong> the interference colour spectrum observed in polarizedlight<br />

microscopes (such as the colour charts <strong>of</strong> Zeiss and Leica). Nevertheless, some<br />

weaknesses in colour reproduction are evident like the second-order green which is far <strong>to</strong>o<br />

prominent in most colour charts. The Michel-Lévy colour chart <strong>of</strong> this <strong>guide</strong> provides an<br />

improved reproduction <strong>of</strong> the colour spectrum (Fig. 4-29). It has been calculated by Dr. Bjørn<br />

Eske Sørensen (Department <strong>of</strong> Geology and Mineral Resources Engineering, NTNU-Trondheim,<br />

Norway) using MATLAB. The spectrum has been calculated taking in<strong>to</strong> account human<br />

colour perception (CIE-calibrated curves <strong>of</strong> sensitivity for the primary colours red, green,<br />

blue) and the RGB colour space <strong>of</strong> the computer. Conformity between calculated and<br />

observed interference colours was further optimised using the gamma correction <strong>of</strong> the<br />

intensity values.<br />

When redesigning the Michel-Lévy colour chart for this <strong>guide</strong> we modified the presentation<br />

such that the application principle for mineral determination is easy <strong>to</strong> grasp, compared with<br />

the widely distributed standard charts <strong>of</strong> Zeiss or Leica (Figs. 4-29 and 4-32). Guided by our<br />

experience with <strong>microscopy</strong> courses we also developed an alternative concept for the<br />

interference colour chart. We believe this chart is easier <strong>to</strong> read where birefringence values or<br />

crystal thickness need <strong>to</strong> be determined, compared with the classic colour chart. The difference<br />

<strong>to</strong> the standard Michel-Lévy colour chart is that the colours representing retardation are<br />

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