2012 - Washington Red Raspberry Commission

More documents

Recommendations

Info

methods are more recent (Bilodeau et al., 2012, Duressa et al., 2012, Markakis et al., 2009). For the culture-based method, plant samples are surface-disinfested with a 10% bleach solution, cut into segments, and then plated into petri dishes containing a semi-selective medium from which diagnostic V. dahliae cultures will grow if the pathogen is present. Soil samples will be dried for 2 weeks to eliminate competing contaminant fungi and then spread on the same semi-selective medium used above. For the DNA-based method, V. dahliae DNA will be extracted and amplified from plants and soil samples using established protocols (Bilodeau et al., 2012, Duressa et al., 2012, Markakis et al., 2009). Pathogen incidence for each site (diseased and nondiseased) will be determined by the presence or absence of V. dahliae as detected by each method. Quantification of the pathogen from plant samples will be based on the number of plants from which the pathogen was detected by each method. Quantification of the pathogen from soil will be based on the number of V. dahliae cultures that grow from each soil sample or the amount of V. dahliae DNA amplified by the DNA-based method. Data will be compiled and results from both diseased and nondiseased sites will be compared to determine the incidence of V. dahliae in red raspberry production fields (Objective 1). Results from plant and soil samples at diseased sites will be used to determine if the presence of V. dahliae in the soil is associated with disease (Objective 2) and to determine which inoculum levels are associated with disease symptoms (Objective 3). Finally, results from each method will be compared to determine which method is best at detecting and at quantifying V. dahliae from plant and soil samples (Objective 4). Incidence data will be analyzed using the chi-square test for independence and quantity data will be analyzed using ANOVA. Timeline: Activity 2013 2014 Plant and soil sample collection June-October June-October Plant and soil sample processing June-December June-December Anticipated Benefits and Information Transfer: This research will identify if V. dahliae is widely prevalent and causing disease in Washington red raspberry production fields. It will also identify inoculum levels that are associated with disease and identify appropriate methods for V. dahliae detection and quantification. Results will lay the foundation for future research including disease screen assays for resistance among red raspberry cultivars and the evaluation of production (e.g. fertilization, irrigation, etc.) and disease management practices (fumigant rates, chemistries, etc.) for their effects on inoculum levels and disease development. Findings will also help growers interpret results from diagnostic laboratories. Our research results will be presented to red raspberry growers at meetings (Small Fruit Conference, Lynden) and communicated to the Washington Red Raspberry Commission, Peerbolt Crop Management, and Whatcom County Extension for inclusion in their newsletters. 107
References: Berkeley, G. H. and Jackson, A. B. 1926. Verticillium wilt of the red raspberry. Scientific Agriculture 6: 261-270. Bilodeau, G. J., Koike, S. T., Uribe, P., and Martin, F. N. 2012. Development of an assay for rapid detection and quantification of Verticillium dahliae in soil. Phytopathology 102: 331-343. Butterfield, E. J. and DeVay, J. E. 1977. Reassessment of soil assays for Verticillium dahliae. Phytopathology 67: 1073-1078. Duressa, D., Rauscher, G., Koike, S., Mou, B., Hayes, R. J., Subbarao, K. V., and Klosterman, S. J. 2012. A real-time PCR assay for detection and quantification of Verticillium dahliae in spinach seed. Phytopathology 102: 443-451. Fiola, J. A. and Swartz, H. J. 1994. Inheritance of tolerance to Verticillium albo-atrum in raspberry. HortScience 29: 1071-1073. Harris, R. V. 1925. The blue stripe wilt of the raspberry. The Journal of Pomology and Horticultural Science 4: 221-229. Markakis, E. A., Tjamos, S. E., Antoniou, P. P., Paplomatas, E. J., and Tjamos, E. C. 2009. Symptom development, pathogen isolation and real-time qpcr quantification as factors for evaluating the resistance of olive cultivars to Verticillium pathotypes. European Journal of Plant Pathology 124: 603-611. Figure 1. Increasing severity of verticillium wilt symptoms (from left to right) on inoculated plants of ‘Meeker’ red raspberry in a 2012 greenhouse disease assay. A) healthy, noninoculated control plant. B) less vigorous growth of inoculated plant. C-E) stunted growth, wilting, cane death, and leaf yellowing on inoculated plants. F) entire plant died as a result of inoculation. Picture was taken two months after inoculation with V. dahliae. 108
Page 1 and 2:
2013 Research Proposals and 2012 Re
Page 3 and 4:
Table of Contents Primary Investiga
Page 5 and 6:
2012 WASHINGTON RED RASPBERRY COMMI
Page 7 and 8:
ut the effect was short lived (Pink
Page 9 and 10:
Privѐ, J., J.A. Sullivan, and J.T.
Page 11 and 12:
Corbin, A Benedict, C Cogger, C Bar
Page 13 and 14:
Page 15 and 16:
Budget: Indirect or overhead costs
Page 17 and 18:
Justification and Background: In 20
Page 19 and 20:
Budget: Indirect or overhead costs
Page 21 and 22:
2012 PROGRESS REPORT Title: Red Ras
Page 23 and 24:
than most other varieties but are s
Page 25 and 26:
Table 1. Yield and fruit weight of
Page 27 and 28:
Table 3. Harvest data for hand-harv
Page 29 and 30:
Table 5. Susceptibility (S) and res
Page 31 and 32:
identifying new, unbroken sources o
Page 33 and 34:
Page 35 and 36:
tested rigorously over a period of
Page 37 and 38:
Other funding sources: All amounts
Page 39 and 40:
Page 41 and 42:
We will collect large quantities of
Page 43 and 44:
Current & Pending Support NAME (Lis
Page 45 and 46:
Appendices Table RY1. Red raspberry
Page 47 and 48:
Table RY3. Mean yield and berry siz
Page 49 and 50:
Table RY5. Mean yield and berry siz
Page 51 and 52:
8 Table RY8. Ripening season for pr
Page 53 and 54:
- Cultivars for the Pacific Northwe
Page 55 and 56:
Current & Pending Support Chad Finn
Page 57 and 58: Washington Red Raspberry Commission
Page 59 and 60: Title of project: Support of SCRI P
Page 61 and 62: We will develop markers that will b
Page 63 and 64: the operations amenable to and desi
Page 65 and 66: Budget: Indirect or overhead costs
Page 67 and 68: Current & Pending Support-Tarara In
Page 69 and 70: Table 1. Primocane injury and weed
Page 71 and 72: Importantly, floricane injury and b
Page 73 and 74: 2013 WASHINGTON RED RASPBERRY COMMI
Page 75 and 76: density. The roots and shoots are s
Page 77 and 78: Name (List PI #1 first) Moore, P.P.
Page 79 and 80: Publications/Presentations: July 18
Page 81 and 82: Table 2. 2011 and 2012 harvest of 2
Page 83 and 84: PROJECT: 13C-3755-5641 TITLE: Red R
Page 85 and 86: PROPOSED BUDGET: Funds from the Nor
Page 87 and 88: Washington Red Raspberry Commission
Page 91 and 92: • Maximum number of plants per se
Page 93 and 94: Project Title: Fungicide Resistance
Page 95 and 96: society’s/the environment’s bes
Page 97 and 98: aged, contact/ingestion residues fo
Page 99 and 100: Table 3. Clay color weevil topical
Page 101 and 102: Project No.: New Title: Management
Page 103 and 104: growers in the Lynden area that hav
Page 105 and 106: Application Date: November 13, 2012
Page 107: particular inoculum levels are asso
Page 113 and 114: technical bulletin states “For pe
Page 115: Budget: 2013 2014 Salaries $0 $0 Ad
show all

2012 - Washington Red Raspberry Commission

Create successful ePaper yourself

Delete template?

Save as template?