2012 - Washington Red Raspberry Commission

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Title: New strategies to control root-lesion nematodes Personnel: Axel Elling, Assistant Professor (Nematology), Department of Plant Pathology, Washington State University, P.O. Box 646430, Pullman, WA 99164-6430 Reporting Period: 2012 Accomplishments: This project depends on the availability of large quantities of pure P. penetrans. The life cycle of this nematode takes 4-6 weeks and its reproductive rate is relatively low, which makes it challenging to mass-produce P. penetrans. We propagated nematodes in the greenhouse, isolated them and manually removed contaminating plant material and other nematodes. In 2012, we produced a sufficient amount of root-lesion nematodes to isolate mRNA. We prepared the mRNA for sequencing and completed the actual high-throughput sequencing step at Washington University St. Louis. At present, we are preparing the sequencing data for further analyses. Results: We have produced enough root-lesion nematodes in the greenhouse to isolate the necessary amount of mRNA for sequencing. Samples were submitted to Washington University St. Louis, where they were sequenced. Preliminary analyses show that the sequencing results are of good quality. We are currently processing the sequencing data (e.g., assembly of small overlapping fragments into longer regions, removal of plant sequences etc.) and will continue to analyze the data to find secretion and effector genes. Publications/outputs: ‘Host-parasite interactions and genomics of plant-parasitic nematodes’, Department of Entomology, Washington State University, Seminar Series, Pullman, WA, September 6 ‘Variability in Meloidogyne chitwoodi and the use of effector genes as control targets’, 64 th International Symposium on Crop Protection, Ghent, Belgium, May 22 ‘Lessons from a plant-parasitic nematode: genomes and host-parasite relationships’, Department of Biological Sciences, University of Idaho, Seminar Series, Moscow, ID, May 1 037
2013 WASHINGTON RED RASPBERRY COMMISSION RESEARCH PROPOSAL Project Title: New strategies to control root-lesion nematodes Proposed Duration: 3 years PI: Axel Elling Organization: Department of Plant Pathology, Washington State University Title: Assistant Professor (Nematology) Phone: 509-335-3742 Email: elling@wsu.edu Address: P.O. Box 646430, Pullman, WA 99164-6430 Year Initiated 2011 Current Year 2013 Terminating Year 2013 Total Project Request: Year 1 $7,288 Year 2 $7,320 Year 3 $7,457 Other funding sources: Agency Name: Washington Wheat Commission Amt. Requested: $37,500 Notes: WSU is including this information on other resources available for the support of similar research undertaken by the faculty member proposing this research. These resources are listed to identify other support granted for this research and are not included as a commitment of costshare by the institution. Description: The objective of this project is to identify nematode genes that allow root-lesion nematodes (Pratylenchus spp.) to infect raspberries and to establish themselves as parasites. Plant-parasitic nematodes have evolved highly sophisticated interactions with their hosts that are based on secretions from the nematode’s salivary glands. These secretions (i.e., effectors) are released into the plant during the infection process and enable the nematode to overcome plant defense responses. We will identify nematode genes that produce the relevant secretions (i.e., effector genes). Specific outcomes of this project will be a list of root-lesion nematode genes that most likely play key roles in the infection process and that make attractive new targets for molecular control strategies. Additionally, we will gain a better understanding of how root-lesion nematodes infect plants and how the infection process can be interrupted. Justification and Background: The root-lesion nematode Pratylenchus penetrans is a widespread and economically important pest in Washington’s raspberry production areas (McElroy 1992). Current nematode control strategies strongly depend on pre-planting applications of synthetic nematicides, but recent decisions by the EPA make it likely that fewer nematicides will be available going forward. Nonfumigant nematicides are always under regulatory threat because they are generally older, 038
Page 1 and 2: 2013 Research Proposals and 2012 Re
Page 3 and 4: Table of Contents Primary Investiga
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Page 7 and 8: ut the effect was short lived (Pink
Page 9 and 10: Privѐ, J., J.A. Sullivan, and J.T.
Page 11 and 12: Corbin, A Benedict, C Cogger, C Bar
Page 15 and 16: Budget: Indirect or overhead costs
Page 17 and 18: Justification and Background: In 20
Page 21 and 22: 2012 PROGRESS REPORT Title: Red Ras
Page 23 and 24: than most other varieties but are s
Page 25 and 26: Table 1. Yield and fruit weight of
Page 27 and 28: Table 3. Harvest data for hand-harv
Page 29 and 30: Table 5. Susceptibility (S) and res
Page 31 and 32: identifying new, unbroken sources o
Page 35 and 36: tested rigorously over a period of
Page 37: Other funding sources: All amounts
Page 41 and 42: We will collect large quantities of
Page 43 and 44: Current & Pending Support NAME (Lis
Page 45 and 46: Appendices Table RY1. Red raspberry
Page 47 and 48: Table RY3. Mean yield and berry siz
Page 49 and 50: Table RY5. Mean yield and berry siz
Page 51 and 52: 8 Table RY8. Ripening season for pr
Page 53 and 54: - Cultivars for the Pacific Northwe
Page 55 and 56: Current & Pending Support Chad Finn
Page 57 and 58: Washington Red Raspberry Commission
Page 59 and 60: Title of project: Support of SCRI P
Page 61 and 62: We will develop markers that will b
Page 63 and 64: the operations amenable to and desi
Page 67 and 68: Current & Pending Support-Tarara In
Page 69 and 70: Table 1. Primocane injury and weed
Page 71 and 72: Importantly, floricane injury and b
Page 75 and 76: density. The roots and shoots are s
Page 77 and 78: Name (List PI #1 first) Moore, P.P.
Page 79 and 80: Publications/Presentations: July 18
Page 81 and 82: Table 2. 2011 and 2012 harvest of 2
Page 83 and 84: PROJECT: 13C-3755-5641 TITLE: Red R
Page 85 and 86: PROPOSED BUDGET: Funds from the Nor
Page 87 and 88: Washington Red Raspberry Commission
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2013 WASHINGTON RED RASPBERRY COMMI
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• Maximum number of plants per se
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Project Title: Fungicide Resistance
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society’s/the environment’s bes
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aged, contact/ingestion residues fo
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Table 3. Clay color weevil topical
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Project No.: New Title: Management
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growers in the Lynden area that hav
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Application Date: November 13, 2012
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particular inoculum levels are asso
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References: Berkeley, G. H. and Jac
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2013 WASHINGTON RED RASPBERRY COMMI
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technical bulletin states “For pe
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Budget: 2013 2014 Salaries $0 $0 Ad
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2012 - Washington Red Raspberry Commission

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