2009 Abstracts - Association for Chemoreception Sciences

More documents

Recommendations

Info

trends in expression of GAP-43, OMP and G a/o . Expression wasreduced to 58%, 60%, and 43%, respectively, but was notsignificantly different from WT, probably due to a small samplesize (n = 3 for p53 -/- ). We observed similar results of GAP-43 andOMP (p
Across all odorants and the 2 presentation conditions, as well asacross the odorants for just retronasal and for just the oral-cavityonly(OCO) presentations, there were significant differences in %correct ID, p 65%) but not OCO (≤ 33%), with OCO geraniol ID at 0%.For 4 of the 6 odorants, retronasal median % correct ID werelarger than corresponding OCO median % correct ID. Within thestudied set of odorants, ID of dl-menthol was difficult.CONCLUSIONS: TRPM8 agonists do not share a commonodorant ID and are responded to differently both betweenretronasal versus OCO presentations and for OCO per se.The OCO differences suggest that TRM8 classification is notsufficient to predict trigeminal responses to odorants. Supportfrom the Food Science Summer Scholars Program, the BiologicalSciences Honors Program, and a Susan Lynn Sage Professorship#P254 Poster session VI: Chemosensory developmentand Psychophysics IEph/Ephrin Expression in the Developing and AdultTaste SystemGennadiy Katsevman, Michael Oleksiak, Natalia Hoshino, MWilliam RochlinLoyola University Chicago Chicago, IL, USAWe are profiling the expression of Eph receptors and their Ephrinligands in the taste system of embryonic to adult mouse and rat.Both classes of protein can initiate signaling cascades that underliecontact dependent repulsion or adhesion, thereby influencingaxon pathfinding, target selection, and epithelial cell arrangement.“Forward” signaling refers to signaling within the Eph-bearingcell; “reverse” signaling is for Ephrin-bearing cells. In E11.5 and14.5 mouse, EphrinB1 is detected in mandibular and chordatympani axons that are bordered by EphB2-positive and EphB3-positive cells, respectively. Thus, reverse signaling could constrictthese axons to their paths. In the tongues of embryonic mouse/ratand postnatal rat, however, anti-EphB1,2,3 labels geniculate andtrigeminal axons; and Ephrins B1 and B2 are most concentrated inthe non-gustatory epithelium avoided by these axons (mouseonly). These data are consistent with a role for forward signalingin restricting the exploration of sensory axons in the target.EphA4, EphA7, and EphrinA2 are also present in embryonicmouse geniculate and trigeminal nerves. We have not detectedEph receptors or ephrins in adult mouse/rat sensory nerves, butboth classes are detected in other adult tissues: In mouse,EphrinB1 and B2 staining of non-gustatory epithelium persists,and EphrinB1 is also detected at low levels within taste buds.In rat fungiform papillae, anti-EphA4 labels taste buds and anti-EphA7 appears to label microvasculature. These results areconsistent with a role for Eph/ephrin signaling in axon guidanceand targeting during development and plasticity, and perhapsduring recovery following injury. In vitro functional assays areunderway to determine if Eph/ephrin signaling supports orinhibits sensory axons throughout development.#P255 Poster session VI: Chemosensory developmentand Psychophysics IInvestigation of detection and pain thresholds at different sitesat the human nasal mucosa in response to electrical stimuliMandy Scheibe, Annika SchmidtSmell & Taste Clinic, Department of Otorhinolaryngology,University of Dresden Medical School Dresden, GermanyBackground: Previous investigations in humans suggestdifferences in the distribution of intranasal trigeminalchemosensitivity. The aim of the present study was to investigatethese topographical differences in the human nasal mucosa usingunspecific electrical stimuli. Material and Methods: A total of 27young, healthy volunteers participated (11 men, 16 women; age22-30 years). Detection and pain threshold of trigeminal stimuliwere investigated at 5 different sites at the nasal mucosa: anteriorseptum, posterior septum, lower turbinate, middle turbinate andanterior lateral nasal wall. Electrical stimuli were applied with aspherical electrode. Results: At the anterior parts of the nosesignificantly higher trigeminal sensitivity was found than at theposterior part. There was a similar distribution pattern of thesensitivity for detection and pain thresholds. Conclusions: Thepresent data suggest that there are topographical differences in thedistribution of trigeminal receptors at the human nasal cavity.Thereby it seems that the highest sensitivity is located in theanterior part. This finding is compatible with the idea that thetrigeminal system acts as a sentinel of the human airways withregard to toxic agents.#P256 Poster session VI: Chemosensory developmentand Psychophysics ITime-Intensity ratings of nasal irritation from pulsedhomologous alcoholsPaul M Wise, Kai Zhao, Charles J WysockiMonell Chemical Senses Center Philadelphia, PA, USARelatively few studies have focused on how nasal irritationchanges over time. To simulate the rhythm of natural respiration,subjects received 3-sec pulses of VOC (volatile organiccompound) interspersed with 3-sec pulses of clean air. Each trial,subjects received nine VOC pulses over 51 sec. Subjects ratednasal irritation from each pulse using magnitude estimation.Within a trial, compound and concentration were fixed.Compound (n-ethanol, n-butanol, and n-hexanol) andconcentration (four levels for each compound) varied across trials.Findings: 1) For all stimuli, rated irritation decreased over time(adaptation). Plots of log rated intensity versus elapsed time wereapproximately linear (intensity decreased by a fixed ratio per unittime). Interestingly, the slopes of intensity versus time functionsdiffered very little: Regardless of concentration and compound,rated irritation decreased by about 30 or 40% over the ninepulses. 2) For a given compound, the slopes of intensity versusconcentration (psychophysical) functions remained constantacross pulses. Overall intensity decreased over time, but the106 | AChemS Abstracts 2009
Page 3 and 4:
AChemSAssociation for Chemoreceptio
Page 5 and 6:
Page 7 and 8:
Page 9 and 10:
#4 GustationGPR40 knockout mice hav
Page 11 and 12:
small population of cells respondin
Page 13:
higher order areas. The beta oscill
Page 17 and 18:
conclusions limited, however, by th
Page 19 and 20:
expressed in the taste cells may al
Page 21:
glomerulus varies across individual
Page 24 and 25:
TH/GFP expression levels in depolar
Page 26 and 27:
not activation and sensitivity. Fur
Page 28 and 29:
POSTER PRESENTATIONS#P1 Poster sess
Page 30 and 31:
and gender (all male). Our results
Page 32 and 33:
activation in psychiatric disorders
Page 34 and 35:
the e4 allele. The ApoE e4 allele i
Page 36 and 37:
including the olfactory epithelium,
Page 38 and 39:
and posterior (MeP), which are diff
Page 40 and 41:
75 and 39 of 80 PbN cells were acti
Page 42 and 43:
on the left side and from 60.9 ± 1
Page 44 and 45:
#P52 Poster session II: Chemosensor
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
esponses (net spikes) evoked by app
Page 54 and 55:
These findings demonstrate the capa
Page 56 and 57: ecorded units tracked stimuli up to
Page 58 and 59: elationship in the characteristic r
Page 60 and 61: #P103 Poster session II: Chemosenso
Page 62 and 63: #P108 Poster session III: Cortical
Page 66 and 67: luciferase-based reporter gene assa
Page 72 and 73: 1987). MP’s olfactory discriminat
Page 76 and 77: discriminate between the H 2 S/IAA
Page 78 and 79: #P160 Poster session IV: Chemosenso
Page 80 and 81: subject to native regulatory mechan
Page 84 and 85: G protein-coupled receptors for bit
Page 92 and 93: eta, ENAC gamma), b-actin, PLC-b 2
Page 94 and 95: presented in a recognition memory p
Page 96 and 97: #P217 Poster session V: Chemosensor
Page 98 and 99: educed granule cell spiking. These
Page 100 and 101: #P230 Poster session V: Chemosensor
Page 102 and 103: data here from mouse studies using
Page 104 and 105: in taste bud induction and developm
Page 108 and 109: elationship between concentration a
Page 110 and 111: four (4 AFC) that they believe is m
Page 112 and 113: #P268 Poster session VI: Chemosenso
Page 114 and 115: pleasantness (r=.275 p=.006), where
Page 116 and 117: utyl, hexyl, and octyl benzene). We
Page 118 and 119: taller compared to wild-type mice.
Page 120 and 121: animals over the age of P24 were gi
Page 122 and 123: classify subjects as PROP non-taste
Page 124 and 125: al 2008. Increases in glucose sensi
Page 126 and 127: #P315 Poster session VII: Chemosens
Page 128 and 129: differences in taste receptors is n
Page 130 and 131: IndexAbaffy, T - 48Abakah, R - P299
Page 132 and 133: Illig, K - 19, P109Imoto, T - P136I
Page 134 and 135: Rucker, J - P305Rudenga, K - P315Ru
Page 136 and 137: AChemS Abstracts 2009 | 135
Page 138 and 139: Registration7:30 am to 1:00 pm, 6:3
Page 140 and 141: Notes______________________________
Page 142 and 143: See you next yearat ournew venue!Tr
show all

2009 Abstracts - Association for Chemoreception Sciences

Create successful ePaper yourself

Delete template?

Save as template?