2009 Abstracts - Association for Chemoreception Sciences

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esponses (net spikes) evoked by applying ethanol (3, 5, 10, 15, 25and 40% v/v) and standard stimuli (including [in M] 0.5 sucrose,0.1 NaCl, 0.01 HCl and 0.01 quinine) to the tongue and palatewere recorded from single neurons in nucleus tractus solitarius.Neurons were also tested with a concentration series of sucrose(0.01, 0.03, 0.1, 0.3 and 1 M) and various other stimuli (fructose,glucose, a sucrose/quinine mixture and individual components ofthis mixture, KCl, citric acid, NaNO 3 , nicotine and MgCl 2 ). Thusfar, 14 neurons have been recorded from P rats and 6 cells fromNP rats. Preliminary analyses reveal that ethanol produces salient,concentration-dependent activation of NTS cells in both P andNP (F 5,90 = 24.48, P
induced by intranasal application of 5% ZnSO 4 with 5%lidocaine. Then, conditioned taste aversion (CTA) to 10% ethanolwas induced using LiCl, and testing in the gustometer wasrepeated. In separate groups of control and anosmic mice longtermconsumption of ethanol solutions was assessed in 48-htwo-bottle test. We have shown that 10% and stronger ethanolsolutions were avoided in both long-term and brief access tests byintact naïve 129P3/J mice, but not by anosmic mice. This suggeststhat an odor of ethanol is a principle signal for ethanol avoidancein both brief-access and long-term tests. Anosmia did not changeunconditioned licking responses to aversive quinine and palatablesucrose, and did not interfere with development of CTA toethanol. Our data suggest that mice use both gustation andolfaction for chemosensory detection of ethanol, and thatolfactory input plays the primary role in ethanol avoidance.#P80 Poster session II: Chemosensory response to,and control of, feeding/NeuroethologyMaillard Reacted Peptides (MRPs) Modulate Benzamil (Bz)-insenstive NaCl Chorda Tympani (CT) Taste Nerve Responsesand Blood Pressure (BP) in Dahl Salt-sensitive RatsShyama Masilamani 1 , Jamison Coleman 2 , Pamela Melone 2 ,Shobha Mummalaneni 2 , Tadayoshi Katsumata 3 , John ADeSimone 2 , Vijay Lyall 21Department of Medicine Division of Nephrology VCURichmond, VA, USA, 2 Department of Division of Physiology andBiophysics VCU Richmond, VA, USA, 3 Kyowa hakko Food Sp.Co. LTD Ibaraki, JapanMRPs conjugated with galacturonic acid (GalA-MRP) producebiphasic effects on the rat Bz-insensitive NaCl CT responses.At low concentrations (0.1-0.25%) GalA-MRP enhances and athigh concentrations (0.3-1.5%) inhibits the Bz-insensitive NaClCT response. The maximum enhancement and inhibition of theNaCl CT response was obtained at 0.25% and 1.5% GalA-MRP.We hypothesize that at these concentrations GalA-MRP can beused as a salt taste enhancer and a salt taste suppressor,respectively. Accordingly, the effect of 0.25% and 1.5% GalA-MRP was tested on BP in Dahl salt-sensitive rats fed a dietcontaining 0.3% NaCl. Radio-telemetry blood pressuretransmitters were surgically implanted in rats to monitor theirmean arterial pressure (MAP) in conscious, unrestrained rats for15s every 15 min over a 24 hr period. The average daytime (7 AMto 7 PM) and nighttime (7 PM to 7 AM) MAPs were recorded foreach 24 hr period. Rats maintained on rat chow containing 0.3%NaCl demonstrated a slow rise in their nighttime MAP over a 2week period (0.5-1.6 mm Hg/day). The spontaneous increase inMAP was inhibited when rats were fed 0.3% NaCl dietcontaining 0.25% GalA-MRP over a period of 4 weeks (0.05-0.17mm Hg/day). Following removal of the MRP from the diet, MAPincreased at the rate of 0.3-2.0 mm Hg/day for 2 weeks. In rats fed0.3% NaCl diet containing 1.5% GalA-MRP the MRP increasedat a rate of 0.89±0.17 mm Hg/day relative to 0.22±0.05 mmHg/day in rats maintained on 0.3% NaCl diet alone (mean±SEM;p
Page 3 and 4: AChemSAssociation for Chemoreceptio
Page 9 and 10: #4 GustationGPR40 knockout mice hav
Page 11 and 12: small population of cells respondin
Page 13: higher order areas. The beta oscill
Page 17 and 18: conclusions limited, however, by th
Page 19 and 20: expressed in the taste cells may al
Page 21: glomerulus varies across individual
Page 24 and 25: TH/GFP expression levels in depolar
Page 26 and 27: not activation and sensitivity. Fur
Page 28 and 29: POSTER PRESENTATIONS#P1 Poster sess
Page 30 and 31: and gender (all male). Our results
Page 32 and 33: activation in psychiatric disorders
Page 34 and 35: the e4 allele. The ApoE e4 allele i
Page 36 and 37: including the olfactory epithelium,
Page 38 and 39: and posterior (MeP), which are diff
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Page 42 and 43: on the left side and from 60.9 ± 1
Page 44 and 45: #P52 Poster session II: Chemosensor
Page 54 and 55: These findings demonstrate the capa
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Page 58 and 59: elationship in the characteristic r
Page 60 and 61: #P103 Poster session II: Chemosenso
Page 62 and 63: #P108 Poster session III: Cortical
Page 66 and 67: luciferase-based reporter gene assa
Page 72 and 73: 1987). MP’s olfactory discriminat
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Page 78 and 79: #P160 Poster session IV: Chemosenso
Page 80 and 81: subject to native regulatory mechan
Page 84 and 85: G protein-coupled receptors for bit
Page 92 and 93: eta, ENAC gamma), b-actin, PLC-b 2
Page 94 and 95: presented in a recognition memory p
Page 96 and 97: #P217 Poster session V: Chemosensor
Page 98 and 99: educed granule cell spiking. These
Page 100 and 101: #P230 Poster session V: Chemosensor
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data here from mouse studies using
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in taste bud induction and developm
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trends in expression of GAP-43, OMP
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elationship between concentration a
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four (4 AFC) that they believe is m
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#P268 Poster session VI: Chemosenso
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pleasantness (r=.275 p=.006), where
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utyl, hexyl, and octyl benzene). We
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taller compared to wild-type mice.
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animals over the age of P24 were gi
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classify subjects as PROP non-taste
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al 2008. Increases in glucose sensi
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#P315 Poster session VII: Chemosens
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differences in taste receptors is n
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IndexAbaffy, T - 48Abakah, R - P299
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Illig, K - 19, P109Imoto, T - P136I
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Rucker, J - P305Rudenga, K - P315Ru
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AChemS Abstracts 2009 | 135
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Registration7:30 am to 1:00 pm, 6:3
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Notes______________________________
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See you next yearat ournew venue!Tr
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2009 Abstracts - Association for Chemoreception Sciences

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