2009 Abstracts - Association for Chemoreception Sciences

#P105 Poster session III: Cortical chemosensoryprocessing/Receptor genomics and molecular biologyAnterior Olfactory Nucleus: A Golgi Study ofDendritic MorphologyPeter C. Brunjes, Michael KenersonUnivesrity of Virginia Charlottesville, VA, USAThe anterior olfactory nucleus (AON) is the first bilaterallyinnervated structure in the olfactory system. It is typically dividedinto “pars principalis“, a thick ring of cells that surrounds theremnant of the olfactory ventricle [usually subdivided into parsmedialis (“pm“), dorsalis (“pd“), lateralis (“pl“) and ventroposterior(“pvp”)] and “pars externa“, a thin ring of cells encirclingthe anterior aspect of the structure. Little is known about theinternal structure of either region. We performed a quantitativeGolgi study to provide the first detailed look at the resident cells.Brains from 8 juvenile rats were stained with the Golgi-Coxmethod and sections counterstained with methyleneblue. Neurolucida ® software was used to reconstruct the cells andsubject them to standard “branch” and “Sholl” analyses. A totalof 206 “pyramidal”-type cells were examined in pars principalis(68 from deep, 71 from middle and 67 from the superficial thirdsof Layer II, and further keyed as to location (19; pm, 73; pd, 86 ;pl, and 28; pvp). Preliminary analyses indicate no deep-superficialdifferences in total dendritic length or number of branches(medians: apical 855µm length and 18 branches, basilar: 17branches, 430 µm length). Two varieties of cells in pars externawere also examined: the typical cell with two apical dendritesextending into Layer Ib (sample = 50 cells: total dendritic length:894 µm) and a second, complex cell with more primary apicaldendrites plus basilar processes (26 cells: total apical length:944 µm, basilar length 605 µm). Other less common cells werealso observed, but due to the lack of a large sample were notsubjected to a quantitative analysis. The results provide importantinformation for understanding and modeling the circuitry ofthe AON#P106 Poster session III: Cortical chemosensoryprocessing/Receptor genomics and molecular biologyDetecting the taste-specific temporal type byfMRI – salty and sweet-Yuko Nakamura 1 , Tazuko K Goto 1 , Kenji Tokumori 1 , TakashiYoshiura 1 , Koji Kobayashi 2 , Yasuhiko Nakamura 2 , HiroshiHonda 1 , Yuzo Ninomiya 1 , Kazunori Yoshiura 11Kyushu University Fukuoka, Japan, 2 Kyushu UniversityHospital Fukuoka, JapanTaste perception has a temporal dimension. The sensoryevaluation in humans showed that, for example, the reaction timeis shorter in salty than in that of sweet. The purpose of this studywas to investigate the differences between salty and sweet intemporal neural responses in the human cortex. For this purpose,we used a new temporal model analysis to demonstrate thetemporal parcellation of brain activity by whole brain analysis viafMRI. Healthy volunteers (ten males and ten females, 19-29 yrs ofage) participated in this study, and all images were acquired with a3.0-T MRI. Salty (0.1M sodium chloride) and sweet (0.5Msucrose) were used as tastants and tasteless artificial saliva (25 mMKCl plus 2.5 mM NaHCO 3 ) as the control. Image data analysiswas performed using the SPM5. First, we analyzed fMRI datasetsby using the standard approach model, and confirmed that theactivated areas of both tastants were located on the putativehuman primary taste cortex (uncorrected P