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ADVERSE IMPACTS OF TRANSGENIC CROPS/FOODS :A COMPILATION OF SCIENTIFIC REFERENCES WITH ABSTRACTStreatments, suggesting that intrinsic water-use efficiency (WUE in) might beindistinguishable between Bt and conventional lines. LTNC was higher but P Nmaxwas lower in Bt line compared to conventional line, resulting in significantly lowerphotosynthetic nitrogen-use efficiency (PNUE). This might result from the additionalcost of producing Bt protein in the Bt line due to the effect of competing nitrogenwith photosynthetic machinery. Bt-gene introduction and expression does notsignificantly change WUE inbut may significantly decrease leaf PNUE. Thus wesuggest that Bt rice should be carefully examined in relation to environmentalrisks (e.g. water-body pollution) before planting commercially.10. Boyko A, Molinier J, Chatter W, Laroche A, Kovalchuk I (2010) : Acute butnot chronic exposure to abiotic stress results in transient reduction ofexpression levels of the transgene driven by the 35S promoter. N Biotechnol.27(1): 70-7.The transgenic plant performance depends on the stable expression of theintegrated transgene. In this paper, we have analyzed the stability of the mostfrequently used constitutive promoter, the cauliflower mosaic virus (CaMV) 35Spromoter. We used several independent Nicotiana tabacum lines transgenic forthe luciferase (LUC) or green fluorescence protein (GFP) coding genes driven bythe same 35S promoter. As an indication of the expression level, we measuredthe steady state RNA level, protein level and protein activity. Exposure of plants toan acute single dose of UVC, UVB or X-ray radiation resulted in a decrease of thetransgene expression level, whereas exposure to high temperature increased it.In most of the cases, the expression changed at one to two hours post exposureand returned to normal at four hours. By contrast, plants germinated and grown inthe presence of a low dose of either UVB radiation or CuSO(4) for two weeks didnot show any changes in expression level. We conclude that although theexpression level of the transgenes driven by the 35S promoter can be transientlyaltered by the acute exposure, no substantial changes occur upon constant lowexposure.11. Jiao, Z., Si, X.X., Li, G.K., Zhang, Z.M., Xu, X.P. (2010) : Unintendedcompositional changes in transgenic rice seeds (Oryza sativa L.) studiedby spectral and chromatographic analysis coupled with chemometricsmethods. J. Agric. Food Chem. 58, 1746–1754.Unintended compositional changes in transgenic rice seeds were studied bynear-infrared reflectance, GC-MS, HPLC, and ICP-AES coupled with chemometricsstrategies. Three kinds of transgenic rice with resistance to fungal diseases orinsect pests were comparatively studied with the nontransgenic counterparts interms of key nutrients such as protein, amino acids, fatty acids, vitamins, elements,and antinutrient phytic acid recommended by the Organization for Economic Cooperationand Development (OECD). The compositional profiles werediscriminated by chemometrics methods, and the discriminatory compounds(6)

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