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ADVERSE IMPACTS OF TRANSGENIC CROPS/FOODS :A COMPILATION OF SCIENTIFIC REFERENCES WITH ABSTRACTSproteins. As a part of total protein, the insecticidal protein in plant tissues changesits level through inhibited synthesis, degradation or translocation to developingplant parts, particularly under environmental stresses, thus being closelycorrelated to N metabolism. It can be concluded that developing new cottonvarieties with more powerful resistance, applying certain plant growth regulators,enhancing intra-plant defensive capability, and maintenance of general health ofthe transgenic crop are important in realizing the full transgenic potential in Btcotton.http://onlinelibrary.wiley.com/doi/10.1111/j.1439037X.2006.00240.x/Abstract;jsessionid=0F0C976C5C24DC2D2C63F3A716ED1061.d01t04?deniedAccessCustomisedMessage=&userIsAuthenticated=false20. Nguyen, H.T. & Jehle, J.A. (2007) : Quantitative analysis of the seasonaland tissue-specific expression of Cry1Ab in transgenic maize Mon810. JPlant Dis Protect 114 : 82-87.Tissue specific expression and seasonal abundance of Cry1Ab protiens wereasessed in transgenic Maize plants (Mon 810, ‘Novelis’) from 2 field trial locatednear Bonn and Halle in Germany. A lot of 1085 samples were analysed usingDouble Anti Serum Enzyme Linked Immuno Sorbent Assay (DAS- ELISA):Significant differences in transgene expression levels were noticed betweenidentical commercial varieties of maize when analyzed at different growth stages,in different plant tissues and at two different locations within Germany.21. Kalantidis K, Tsagris M, Tabler M. (2006) : Spontaneous short-rangesilencing of a GFP transgene in Nicotiana benthamiana is possiblymediated by small quantities of siRNA that do not trigger systemic silencing.Plant J. 45(6): 1006-16.A green fluorescent protein (GFP) transgene under the control of the 35Scauliflower mosaic virus (CaMV) promoter was introduced by Agrobacteriummediatedtransformation into Nicotiana benthamiana to generate fourteentransgenic lines. Homozygous lines that contained one or two copies of thetransgene showed great variation of GFP expression under ultraviolet (UV) light,which allowed classification into three types of transgenic plants. Plants frommore than half of the transgenic lines underwent systemic RNA silencing andproduced short interfering RNA (siRNA) as young seedlings, while plants of theremaining lines developed, in a spontaneous manner, defined GFP-silencedzones on their leaves, mostly in the form of circular spots that expanded to about4-7 mm in size. In some of the latter lines, the GFP-silenced spots remainedstable, but no systemic silencing occurred. Here we characterize thisphenomenon, which we term spontaneous short-range silencing (SSRS).Biochemical analysis of silenced spot tissue did not reveal detectable levels ofsiRNA. However, agro-infiltration with the suppressor proteins P19 of cymbidiumring spot virus (CymRSV), HC-Pro of tobacco etch virus (TEV), and crosses to a(12)

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