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GENE FLOW, CONTAMINATION & FIELD TRIALS’ RISK28. Schubbert, R., Rentz, D., Schmitzx, B. and Doerfler, W. (1997) : Foreign(M13 DNA ingested by mice reaches peripheral leukocytes, spleen andliver via the intestinal wall mucosa and can be covalently linked to mouseDNA. Proc. Nat. Acad. Sci. 94, 961-6.Food-ingested foreign DNA is not completely degraded in the gastrointestinaltract of mice. Phage M13mp18 DNA as a test molecule devoid of homology tomouse DNA was pipette-fed to or added to the food supply of mice. The fate of thisforeign DNA in the animals was followed by several methods. In 84 animals,fragments of M13mp18 DNA were detected in the contents of the small intestine,the cecum (until 18 h), the large intestine, or the feces. In 254 animals, M13mp18DNA fragments of up to 976 bp were found in blood 2-8 h after feeding. In bufferfedcontrol animals, M13mp18 DNA could not be detected. M13mp18 DNAfragments were traced by PCR in peripheral leukocytes and located by fluorescentin situ hybridization in about 1 of 1000 white cells between 2 and 8 h, and inspleen or liver cells up to 24 h after feeding, but not later. M13mp18 DNA could betraced by fluorescent in situ hybridization in the columnar epithelial cells, in theleukocytes in Peyer’s patches of the cecum wall, in liver cells, and in B cells, Tcells, and macrophages from spleen. These findings suggest transport of foreignDNA through the intestinal wall and Peyer’s patches to peripheral blood leukocytesand into several organs. Upon extended feeding, M13mp18 DNA could be reclonedfrom total spleen DNA into a lambda vector. Among about 2.5 x 10(7) lambdaplaques, one plaque was isolated that contained a 1299 nucleotide pair fragment(nt 4736-6034) of sequence-identified M13mp18 DNA. This fragment wascovalently linked to an 80 nt DNA segment with 70% homology to the mouse IgEreceptor gene. The DNA from another lambda plaque also contained mouseDNA, bacterial DNA, and rearranged lambda DNA. Two additional plaquescontained M13mp18 DNA fragments of at least 641 (nt 2660-3300) or 794 (nt4640-5433) nucleotide pairs. The medical and evolutionary implications of theseobservations may be considerable.http://www.ncbi.nlm.nih.gov/pubmed/?term=Foreign+%28M13+DNA+ingested+by+mice+reaches+peripheral+leukocytes%2C+spleen+and+liver+via+the+intestinal+wall+mucosa+and+can+be+covalently+linked+to+mouse+DNASteale RE, Hampson SE, Stover NA, Kibler DF and Bode HR (2004) : Probablehorizontal transfer between a protist and a cnidarian. Curr Biol 14(8) : 298-9.Coghlan A (2002) : Does it matter if genes can jump from GM food to bugs inhuman gut? New Scientist. 2353:6.http://www.deepdyve.com/lp/elsevier/probable-horizontal-transfer-of-a-genebetween-a-protist-and-a-s6SF00jjFYhttp://www.newscientist.com/article/mg17523530.400-does-it-matter-if-genes-canjump-from-gm-food-to-bugs-in-human-gut.html(193)