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IMPRECISION & UNPREDICTABILITY OF SCIENCE &TECHNOLOGY OF GENETIC ENGINEERING1. Zhi Liu, Yunhe Li, Jie Zhao, Xiuping Chen, Guiliang Jian, Yufa Peng, andFangjun Qi (2012) : Differentially Expressed Genes Distributed OverChromosomes and Implicated in Certain Biological Processes for SiteInsertion Genetically Modified Rice Kemingdao. Int J Biol Sci. 8(7) : 953–963Release of genetically modified (GM) plants has sparked off intensive debatesworldwide partly because of concerns about potential adverse unintended effectsof GM plants to the agro system and the safety of foods. In this study, with the aimof revealing the molecular basis for unintended effects of a single site insertionGM Kemingdao (KMD) rice transformed with a synthetic cry1Ab gene, and bridgingunintended effects of KMD rice through clues of differentially expressed genes,comparative transcriptome analyses were performed for GM KMD rice and itsparent rice of Xiushui11 (XS11). The results showed that 680 differentiallyexpressed transcripts were identified from 30-day old seedlings of GM KMD rice.The absolute majority of these changed expression transcripts dispersed andlocated over all rice chromosomes, and existed physical distance on chromosomefrom the insertion site, while only two transcripts were found to be differentiallyexpressed within the 21 genes located within 100 kb up and down-stream of theinsertion site. Pathway and biology function analyses further revealed thatdifferentially expressed transcripts of KMD rice were involved in certain biologicalprocesses, and mainly implicated in two types of pathways. One type was pathwaysimplicated in plant stress/defense responses, which were considerably incoordination with the reported unintended effects of KMD rice, which were moresusceptible to rice diseases compared to its parent rice XS11; the other type waspathways associated with amino acids metabolism. With this clue, new unintendedeffects for changes in amino acids synthesis of KMD rice leaves were successfullyrevealed. Such that an actual case was firstly provided for identification ofunintended effects in GM plants by comparative transciptome analysis.2. Love AJ, C Geri, J Laird, C Carr, BW Yun, GJ Loake et al (2012) : CauliflowerMosaic Virus Protein P6 Inhibits Signaling Responses to Salicylic Acidand Regulates Innate Immunity. PLoS One. 7(10) : e47535.Cauliflower mosaic virus (CaMV) encodes a multifunctional protein P6 that isrequired for translation of the 35S RNA and also acts as a suppressor of RNAsilencing. Here we demonstrate that P6 additionally acts as a pathogenicity effectorof an unique and novel type, modifying NPR1 (a key regulator of salicylic acid(SA)- and jasmonic acid (JA)-dependent signaling) and inhibiting SA-dependentdefence responses We find that that transgene-mediated expression of P6 inArabidopsis and transient expression in Nicotiana benthamiana has profoundeffects on defence signaling, suppressing expression of representative SAresponsivegenes and increasing expression of representative JA-responsive(1)