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E-International Scientific Research JournalISSN: 2094-1749 Volume: 2 Issue: 4, 2010typhoons due to <strong>the</strong> El Niňo and La Niňa phenomena. The trend towards <strong>the</strong> “shallowing”process and <strong>the</strong> flooding of rivers due to climate variability can significantly alter <strong>the</strong>conditions of river systems in terms of biodiversity composition and physical condition.Extreme drought causes rivers to dry out and this would mean <strong>the</strong> extinction of some speciesthat are not resistant or adaptable to <strong>the</strong> absence of water (Juranilla-Sanchez et al., 2007). Flashflooding after heavy rains or during typhoons causes <strong>the</strong> river to swell or overflow anddestroys <strong>the</strong> river banks and <strong>the</strong> plants that grow <strong>the</strong>re. Floods also erode <strong>the</strong> soil thatsubsequently contribute to <strong>the</strong> siltation process downstream.Research Method and DesignSeven stations were established as sampling sites along <strong>the</strong> Liboran River and weresampled in June 2006 and May 2010. Physico-chemical parameters such as pH, temperature,dissolved oxygen (DO), salinity, total suspended solids, phosphates and nitrates weremeasured, as well biological parameters such as total coliform, Escherichia coli and planktoncomposition.Determination of Dissolved Oxygen (DO), pH, Salinity and Temperature. In situmeasurements of dissolved oxygen, pH, temperature and salinity were conducted a portableDO meter, pH meter, <strong>the</strong>rmometer and refractometer, respectively in three replications.Phosphates (PO 4 -P mg/L) Analysis. Water samples in three replicates (350 ml each)were taken from <strong>the</strong> three sampling sites and were placed in a glass bottle. (Sampling bottleswere soaked with muriatic acid and washed with a phosphate free detergent before use).Samples were <strong>the</strong>n placed in a styropore box with ice and were brought to Silliman UniversityMarine Laboratory for analysis.Stocks solutions of Sulphuric Acid, Ascorbic Acid, and Mixed Reagent were firstprepared. Sulphuric Acid was prepared through adding 125 ml concentrated H 2 SO 4 to waterand was diluted to 500 ml, stored in a plastic bottle. Ascorbic Acid on <strong>the</strong> o<strong>the</strong>r hand, wasprepared through dissolving 5.0g ascorbic acid in an amber glass bottle with 25 ml water, andadded with 25 ml sulphuric acid solution and stored in <strong>the</strong> refrigerator. Mixed Reagent wasprepared through dissolving 6.25 g (NH 4 ) 6Mo 7 0 24 .4H 2 O in 62.5 ml water. Then 0.25gpotassium antimony tartrate (with or without 1/2H 2 O) was dissolved in 10.0 ml water. Themolybdate solution was <strong>the</strong>n added to 175 ml of dilute sulphuric acid with <strong>the</strong> tartrate, andwas stored in a glass bottle. Then <strong>the</strong> following were added to a test tube or scintillation vial:10 ml filtered sample, 0.2 ml ascorbic acid reagent, and 0.2 ml mixed reagent. Absorbance at880 nm was measured between 5 and 30 minutes. The concentration of PO 4 -P in mg/L is givenby <strong>the</strong> formula of Spotte as quoted by Argente (2009):C u =C s V s A sV u AsW<strong>here</strong>: C u = <strong>the</strong> concentration of <strong>the</strong> unknown in mg PO 4 -P/LC s = <strong>the</strong> concentration of <strong>the</strong> standard in mg PO 4 -P /LA u = <strong>the</strong> absorbance of <strong>the</strong> unknownA s = <strong>the</strong> absorbance of standard367

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