PROFESSOR TYNDALL. I29needle-dip from an already affected tube the resulting contagion was almostimmediate, while where dust was supplied in its dry form, two days mostlyelapsed before any indication of such inoculation made its appearance. Thesagacious explanation of these phenomena, given by Professor Tyndall, wasthat the dust supplied contained only germs in a desiccated state, whichnecessarily required a set time, or "period of latency," to expire beforethey displayed their vital properties while in those taken from the fluid;medium, these vital properties were already in their full force, permittingthe organisms to increase and multiply from the first moment of theircontact with the sterile liquid.One interesting experiment bearing upon the phenomenonlast describedrequires mention. A certain mineral solution, containing in proper proportionsall the substances which enter into the composition of Bacteria, wasfound after inoculation with the least speck of liquid containing livingBacteria, to be always swarming and turbid with such organisms within aspace of twenty-four hours while a small;pinch of laboratory dust added tothe same fluid, and containing the germs in their desiccated condition,remained in contact with the fluid with impunity for many weeks. Bacteriain their living and moist condition, and those in their desiccated state, werethus shown to possess highly differentiated developmental properties.Another fact of importance, elicited by Professor Tyndall, bears referenceto the want of uniformity in the distribution of bacterial and other germs inany given atmosphere. This was demonstrated through the preparation oflarge trays, contrived to hold as many as from sixty to one hundred tubesof infusions side by side, and on the same level. All of these exposed todust-laden air were infallibly, after a greater or less duration of time, teemingwith living organisms, but the order of their affection or inoculation wasfound to differ considerably, intervals of several days not unfrequentlyelapsing between the inoculation of closely contiguous tubes. A considerabledifference was likewise found to obtain, under such conditions, in thecharacter of the developed matter, Bacteria of different species, fungoidgrowths, and other organisms, variously and irregularly preponderating.Professor Tyndall happily explains these phenomena by comparing theaerial distribution of microscopic germs to the cloud-patchesmottled sky ;visible in aall parts of the landscape, as represented by the tray of tubes,being overshadowed in turn by these patches, but in no definite or regularsequence. It has been pointed out by Professor Huxley, that a closelycorresponding simile was originally employed by Ehrenberg, who as anexponent of the atmospheric distribution of Infusoria, either as eggs or intheir encysted state, likened the non-uniformity of their occurrence undersuch conditions to irregularly alternating days of sunshine and heavydownpour. As shown already, however, at page 120, the atmospheric germtheory originated with John Harris, more than a century prior even to thetime of Ehrenberg.That the atmosphere inits purest state may be entirely free from organicK
I3 r ->SPONTANEOUS GENERATION.germs, had already been demonstrated by M. Pasteur through exposinginfusions with perfect immunity from infection, to the open air of theMer de Glace, which experiment, with precisely identical results, wasrepeated by Professor Tyndall in the vicinity of the Bel Alp at an elevationof 7000 feet above the sea, in July of the year 1877. Respecting thecapacity of Bacteria and atmospheric germs to resist exposure to abnormalelevations of temperature, he found the widest divergence to obtain inmaterials derived from different sources or in different conditions of vitality.Where the fully developed and vitally active organisms were experimentedon, contact with boiling water, or sometimes a temperature considerablybelow ebullition, was found sufficient to deprive them of life, but where thedesiccated germinal matter was operated on, the results were as a ruleentirely reversed. In some few instances these germs were so tenderas to succumb to boiling for a term of five minutes, or even less, while inextreme cases they were found sufficiently obstinate to survive a similarordeal of no less than eight hours' duration. As regards their respective"death-points," or limit of heat-resistance, Professor Tyndall suggests thatthe infusorial germs of the atmosphere might be conveniently classified under"the following heads : Killed in five minutes ;not killed in five minutes,but killed in fifteen ;not killed in fifteen minutes, but killed in thirty ;notkilled in thirty minutes, but killed in an hour ;not killed in an hour, butkilled in two hours ;not killed in two, but killed in three hours ;not killedin three, but killed in four hours."Several cases of survival after four, five,six, and even eight hours' boiling were met with, and as he further remarks,there is no valid warrant for fixing upon eight hours as the final limit.The germinal dust obtained from long preserved, and thoroughly desiccated,hay was in all instances found to yield the most obstinately resistingmaterial, and the presence of a truss of hay anywhere in the vicinity of thegerminal matter experimented on, always constituted an importantfactorin its reduction by boiling to a condition of sterility. Notwithstanding,however, the great resistant property possessed by a large number of thesegerms, Professor Tyndall has shown that even the most obstinate canbe sterilized or killed if certain precautions are taken in their treatment.These consist of setting aside the infusion containing them, after ebullition,in a warm room for a period of ten or twelve hours, then raisingitagain to,and maintainingit for a short interval at, the boiling point, repeating theprocess with similar intervals of rest several successive times. By thesemeans the germs as they approach their point of final development aresuccessively killed off in the order of their resistance, and the liquid is in theend completely sterilized.The special chambers improvised by Professor Tyndall for the conductof the experiments above recorded recommend themselves so strongly,on account of their simplicity of form and efficiency in action, both forfurther experiments ina similar direction, and for the cultivation of Infusoriagenerally, that an illustration of one constructed to hold six test-
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aoamoa
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"Our little systems have their day,
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TOTHOMAS HENRY HUXLEY, LL.D.,F.R.S.
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viiiPREFACE.experience some disappo
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XPREFACE.ready and valuable assista
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LEEUWENHOEtfS OBSERVATIONS. 3relate
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LEEUWENHOEICS OBSERVATIONS.5spatter
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LEEUWENHOEK'S OBSERVATIONS.Jstopped
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SIfi E. KING, 1693. JOHN HARRIS, 16
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STEPHEN GRA Y, 1696. LEEUWENHOEK, 1
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HENRY BAKER, 1742, 1753.13"Oct. 6th
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O. F. MULLER, 1773-1786. 15ledge of
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EHRENBERG, 1836. 17Notwithstanding
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F. DUJARDIN, 1841. T. VON SIEBOLD,
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FRIEDRICH STEIN, 1849-1854. 21cules
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CLAPAREDE AND LACHMANN, 1858-1860.
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F. STEIN, 1859. R. M. DIES ING, 184
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ANDREW PRITCHARD, 1861. H. JAMES-CL
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DALLINGER AND DRYSDALE, 1873-1875.
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CHAPTER II.THE SUB-KINGDOM PROTOZOA
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AFFINITIES OF THE SPONGIDA. 33ordin
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PRIMARY SUBDIVISIONS A UTHOKS S YST
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AUTHORS PHYLOGENETIC SCHEME. 37DIAG
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FLA CELLA TA -PANTOS TOMA TA ; FLA
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CHOANO-FLAGELLATA; MYCETOZOA. 41acc
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MYCETOZOAj LABYRINTHULIDA. 43From t
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GROUPS PROTISTA AND MONERA. 45of th
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DISTINCTION BETWEEN PROTOZOA AND PR
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( 49 )CHAPTER III.NATURE AND ORGANI
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AUTHORS CLASSIFICATORY TABLE.TABULA
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UNICELL ULAR NA TURE. 5 3dissolutio
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UN1CELL ULAR NA TURE. 5 5of the ent
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CUTICULAR ELEMENTS. 57substance the
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EXCRETED ELEMENTS. 59by the interca
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EXCRETED ELEMENTS. 6 1transparent,
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ENCYSTMENT. 63corresponding type of
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LOCOMOTIVE AND PREHENSILE APPENDAGE
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ORAL APERTURE. 67Oral Aperture or C
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CONTRACTILE VESICLES. 69shadowed. A
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CONTRACTILE VESICLES.71in the major
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NUCLEUS OR ENDOPLAST. 73to indicate
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NUCLEUS OR ENDOPLAST. 75Spirostomit
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NUCLEOLUS OR ENDOPLASTULE. 77with t
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NATURE AND AFFINITIES OF THE SPONGE
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NATURE AND AFFINITIES OF THE SPONGE
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NATURE AND AFFINITIES OF THE SPONGE
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NATURE AND AFFINITIES OF THE SPONGE
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NATURE AND AFFINITIES OF THE SPONGE
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NATURE AND AFFINITIES OF THE SPONGE
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NATURE AND AFFINITIES OF THE SPONGE
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NATURE AND AFFINITIES OF THE SPONGE
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( 195 )CHAPTER VI.SYSTEMS OF CLASSI
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CLASSIFICATION OF THE INFUSORIA.197
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MULLERS CLA SSIPICA TOR Y S YSTEM.
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EHRENBERG'S CLASSIFICATORY SYSTEM.2
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CLASSIFICATORY SYSTEMS OF SIEBOLD A
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CLAPAREDE AND LACHMANWS CLASSIFICAT
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DIESINGS CLASSIFICATORY SYSTEM. 207
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S TEIN'S CLA SSIFICA TOR Y S Ki TEM
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A UTHOKS CLASSIFICA TOR Y S YSTEM.
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A UTHOR'S CLA SSIPICA TOR Y S YSTEM
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A UTHOKS CLASSIPICA TOR Y S YSTEM.
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CLASS FLAGELLA TA. 2 I7more extensi
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GENUS TRYPANOSOMA. 219Trypanosoma s
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GENUS MASTIGAMCEBA . 221The some ha
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;,HAB.GENUS REPTOMONAS. 22$immediat
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ORDER RA DIO-FLA CELLA TA.225Podost
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Body subspherical orGENUS ACTINOMON
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GENUS SPONGASTERISCUS. 229Spongocyc
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Cladomonas.ipidodendrtSpongomonas.D