SOYBEAN AND HEALTH - University of Macau Library

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172Soybean and HealthFig. 4. Effects of daidzein on catecholamine secretion induced by acetylcholine in bovineadrenal medullary cells. Cells (2x10 6 /dish) were incubated with acetylcholine (0.3 mM) inthe presence (●) or absence (○) of various concentrations (0.1-100 μM) of daidzein for 10 minat 37 °C. Catecholamines secreted into the medium were measured, and expressed aspercentage of total catecholamines. Data are means ± SEM of four experiments carried out induplicate. *p
Insights into the Pharmacological Effects of Soy Isoflavones on Catecholamine System 173seems that the genistein concentrations used in the present study are nutritionally (orpharmacologically) relevant.Fig. 5. Effects of various phytoestrogens on [ 3 H]noradrenaline uptake by SK-N-SH cells (A)and COS-7 cells transfected with bovine noradrenaline transporters (B). (A) SK-N-SH cellswere pretreated for 20 min with or without various concentrations (0.01 – 100 μ M) ofgenistein (●), daidzein (■), or coumestrol (▲), and then incubated for another 10 min with[ 3 H]noradrenaline (0.1 μM, 0.1 μCi) in the presence or absence of phytoestrogens (0.01 - 100μM). Results are presented as percentage of control values (62.3 ± 4.2 fmol/10 6 cells/min).Data are means ± SEM from three separate experiments. *p < 0.05, compared with control.(B) The bovine noradrenaline transporter transfected COS-7 cells were pretreated for 20 minwith various concentrations of genistein (0.01 – 100 μM), and then the desipramine-sensitiveuptake of [ 3 H] noradrenaline by the cells was measured. *p < 0.05, compared with control.Data from Toyohira et al. (2010) are modified.From Eadie-Hofstee analysis of [ 3 H] noradrenaline uptake, genistein (10 μM) caused asignificant increase in the maximal velocity (V max ) of noradrenaline transport with littlechange in the Michealis-Menten constant (K m ) value (Fig. 6A). Scatchard analysis of[ 3 H]nisoxetine binding to COS-7 cells transiently transfected with noradrenaline transportershowed that genistein increases the maximal binding (B max ) without any change in thedissociation constant (K d ) (Fig. 6B).
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PrefaceWorldwide, soybean seed prot
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Soybean Products Consumption in the
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20Soybean and HealthWagner, J.D., A
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22Soybean and HealthDifferences in
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24Soybean and Healthfermented chung
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26Soybean and HealthIsoflavones, wh
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28Soybean and HealthThe concentrati
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30Soybean and Health5. Metabolite p
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32Soybean and Healththat various me
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34Soybean and Healtheffects on hypo
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36Soybean and Healthfatty acid oxid
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38Soybean and HealthThe decreased n
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40Soybean and Healththe N-terminus
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42Soybean and HealthYAVE, IVAE, LVA
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44Soybean and Healthrespectively, w
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46Soybean and HealthIn order to des
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48Soybean and Healthpeptides that w
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50Soybean and Healthreactions, new
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52Soybean and HealthGibbs, B.F.; Zo
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54Soybean and HealthKoo, B.; Seong,
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56Soybean and HealthPak, V.V.; Koo,
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58Soybean and HealthBalance between
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60Soybean and HealthAll results are
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62Soybean and Healthfrom 10.01 ± 1
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64Soybean and Healthmeasured in eac
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66Soybean and HealthFig. 2. Time co
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68Soybean and Healthω3D rats switc
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70Soybean and Health3.4.4 Monodesat
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72Soybean and Healthpercentages yie
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74Soybean and Healthinstance, such
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76Soybean and Healthconcerned the c
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78Soybean and HealthMalaisse, W.J.
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80Soybean and Healthflavonoids show
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82Soybean and HealthSupplies (Poole
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84Soybean and Healthal., 2004). For
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86Soybean and HealthABFig. 1. IBD p
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88Soybean and Healthdamage for both
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The Effect of Flavonoids in Soybean
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Effects of Dietary Soybean Trypsin
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Conjugated Linoleic Acid: A Milk Fa
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Page 191 and 192: 9Occurrence of Biogenic Aminesin So
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Biologically Active Molecules from
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11From Soybean Phytosterolsto Stero
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From Soybean Phytosterols to Steroi
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12Genistein Derivatization - From a
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Genistein Derivatization - From a D
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13Soybean Oil: Production Process,
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Soybean Oil: Production Process, Be
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14Beneficial Effects of Bioactive P
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Beneficial Effects of Bioactive Pep
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15Soy Isoflavones as BioactiveIngre
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Soy Isoflavones as Bioactive Ingred
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16Soybean Oil: How Good or How Bad
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Soybean Oil: How Good or HowBad in
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17Anti-Diarrhoeal Aspectsof Ferment
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Anti-Diarrhoeal Aspects of Fermente
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18Antioxidant and Hypocholesterolem
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Antioxidant and Hypocholesterolemic
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19Important Minor Soybens Proteins:
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Important Minor Soybens Proteins: S
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20Soybean Allergens: Presence, Dete
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21Nutritional and Bioactive Compoun
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Nutritional and Bioactive Compounds
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22Polyamines - The Principal Candid
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Polyamines - The Principal Candidat
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