SOYBEAN AND HEALTH - University of Macau Library

246Soybean and Healthconcentrations of steroid hormones, phytosterol uptake capacity, simplicity of genemanipulation, and cell cultivation. This would need to be founded on a comprehensiveunderstanding of the processes by which phytosterols are converted to products, includingthe degradation of C17-side chains. In order to facilitate the assembly and control of thissystem, the process of design and reconstruction should based on synthetic biology,standardizing the gene parts required, designing controllable and efficient gene circuits,developing functional modules, and integrating and optimizing the rebuilt system in theselected host. Therefore, a novel engineered organism with ideal phytosterol-transformingproperties may be developed (Fig. 5A).Fig. 5. Transformation pathway of phytosterols in S. cerevisiae.