5 The role of quorum-sensing in the virulence of Pseudomonas ...

The signs of illness in the mice infected with ∆PAPI-1, ∆PAPI-2 and the wild-type,
PA14 were not too dissimilar at the 18 hours post-infection. The only significant
difference between PA14 and one its isogenic mutants was seen with the double
knockout, ∆PAPI-1∆PAPI-2, where mice were demonstrating little or no signs of
illness at 18 hours.
Symptom score
6
4
2
0
PAO1
PA14
^PAPI-1
104
^PAPI-2
*
^P1^P2
Figure 4-9 Symptom scores at 18 hours post-infection with 2x10 6 CFU/50µl; n=10 for each
strain. Each dot represents a single mouse. All data were generated over 2 independent
experiments for each strain. *denotes significant difference when compared with wild-type
PA14 P