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Characterisation of pathogenicity i
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Statement of Originality This accom
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% Percent Abbreviations ºC Degrees
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Table of contents 1 INTRODUCTION 1
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4 THE CONTRIBUTION OF PAPI-1 AND PA
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1 Introduction 1 INTRODUCTION 1 1.1
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Cell-surface Proteases Haemolysins
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1.2.1 Pathogenicity islands: a subg
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appear to be related and there have
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assessed in number of assays and mo
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TAR cloning is a method designed by
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Figure 1-3 Top plate shows colony g
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Figure 1-5 depicts the principles o
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Balb/c mice. The models described b
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urns-sepsis model, but not in the n
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ORF ID Gene name Gene function PA14
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functional activities it could be s
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Figure 1-6 Schematic showing the OR
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RhlI Autoinducer RhlR Rhl Regulon G
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1.5.2 Pseudomonas aeruginosa LES Th
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2 Material and Methods 2 MATERIAL A
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2.1.2 Strains used Pseudomonas aeru
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Plasmids Plasmid Description Refere
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In the case of capture vector const
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Primer name Sequence Capture vector
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The relevant homology sequence was
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Important to the use of the capture
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2.3.4 Electroporation: Transfer of
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2.4.2 Infection dose preparation Th
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mould was then added to the metal c
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3 Analysis of genomic islands captu
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3.1 Capture experiments involving g
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DNA No of colonies Control No DNA 0
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were designed based on the assumpti
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3.2.1 KR115-lys10 and KR159-lys10 A
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estriction digestion with I-SceI to
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generated. The most significant nuc
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they both produced three fragments
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elated genomic islands and this res
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All the Yersinia species available
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Leicester). E105-leuX was the first
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The strains highlighted with alignm
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predict this protein to be a dnaJ-c
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Figure 3-15 depicts the codon usage
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ORF Gene range Blastx Description y
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ecognition sequences are found with
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(S: 150, E: 1e-37). This predicts t
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having multiple copies of itself. A
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The capture vectors used during thi
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cloning and their findings was inco
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The genomic island capture method c
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unpublished). This was achieved by
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Pseudomonas aeruginosa PA14 (UCBPP-
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stressful’ to the bacteria and th
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log CFU/mg 4 3 2 1 0 0 2 4 6 8 Days
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mice exhibited visible symptoms at
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4.4.1 Survival times post-infection
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(P
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4.4.4 Intravenous infection A quest
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Symptom score Symptom score Symptom
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- Page 127 and 128: Figure 4-19 Leukocytes numbers moni
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- Page 135 and 136: The intravenous sepsis model data s
- Page 137 and 138: in PAO1(Lee et al. 2006). They show
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- Page 141 and 142: 5 The role of quorum-sensing in the
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- Page 145 and 146: Quorum-sensing is bacterial cell de
- Page 147 and 148: Interestingly, the quorum-sensing d
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- Page 151 and 152: LES431 LESB58 LESB65 LES400 � �
- Page 153 and 154: Additional comparisons of the CFU r
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- Page 171: The data presented in this thesis s
- Page 175 and 176: 6 Thesis conclusion The underlying
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- Page 179 and 180: 1 2 3 4 5 6 7 8 9 10 11 12 13 λHin
- Page 181 and 182: AQ: 1 AQ: 2 AQ: 3 AQ: 4 AQ: 5 AQ: 6
- Page 183 and 184: AQ: 18 AQ: 19 AQ: 20 F1 AQ: 21 tapr
- Page 185 and 186: AQ: 32 AQ: 33 AQ: 34 tapraid5/z9d-j
- Page 187 and 188: AQ: 40 AQ: 41 DISCUSSION tapraid5/z
- Page 189 and 190: AQ: 48 tapraid5/z9d-jid/z9d-jid/z9d
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- Page 193 and 194: 8 Bibliography AL-ALOUL, M., CRAWLE
- Page 195 and 196: D'ARGENIO, D.A., WU, M., HOFFMAN, L
- Page 197 and 198: genomic islands in Pseudomonas aeru
- Page 199 and 200: MURRAY, A.W. and SZOSTAK, J.W., 198
- Page 201 and 202: specificity of chaperone-usher mach
- Page 203 and 204: VAN HEECKEREN, A.M. and SCHLUCHTER,