5 The role of quorum-sensing in the virulence of Pseudomonas ...

More documents

Recommendations

Info

LES431 LESB58 LESB65 LES400 N/A � � LES431 N/A N/A LESB58 � Table 5-6 Significant differences in CFU recovered from the lungs at 72 and 96 hours post- infection. P
log CFU/mg log CFU/mg log CFU/ml 5 4 3 2 1 Nasopharnyx 0 0 12 24 36 48 Hours 60 72 84 96 5 4 3 2 1 Lungs 0 0 12 24 36 48 Hours 60 72 84 96 3 2 1 Blood 0 0 12 24 36 48 Hours 60 72 84 96 145 LES400 LES431 LESB58 LESB65 LES400 LES431 LESB58 LESB65 LES400 LES431 LESB58 LESB65 Figure 5-10 Nasopharynx, lung and blood CFU recovered at 18, 48, 72 and 96 hours post- infection with 2x10 6 CFU; n=10 for 18 and 96 hours post-infection and n=4 for 48 and 72 hours post-infection. Each dot represents the mean log CFU/mg for nasopharynx and lungs and the mean log CFU/ml of blood. The 18 and 96 hour timepoint data were generated over two independent experiments and the 48 and 72 hour data were generated over a single experiment. With the exception of LESB65 96 hour data that were generated in a single experiment. The error bar represents the standard error of the mean.
Page 1 and 2:
Characterisation of pathogenicity i
Page 3 and 4:
Statement of Originality This accom
Page 5 and 6:
% Percent Abbreviations ºC Degrees
Page 7 and 8:
Table of contents 1 INTRODUCTION 1
Page 9 and 10:
4 THE CONTRIBUTION OF PAPI-1 AND PA
Page 11 and 12:
1 Introduction 1 INTRODUCTION 1 1.1
Page 13 and 14:
Cell-surface Proteases Haemolysins
Page 15 and 16:
1.2.1 Pathogenicity islands: a subg
Page 17 and 18:
appear to be related and there have
Page 19 and 20:
assessed in number of assays and mo
Page 21 and 22:
TAR cloning is a method designed by
Page 23 and 24:
Figure 1-3 Top plate shows colony g
Page 25 and 26:
Figure 1-5 depicts the principles o
Page 27 and 28:
Balb/c mice. The models described b
Page 29 and 30:
urns-sepsis model, but not in the n
Page 31 and 32:
ORF ID Gene name Gene function PA14
Page 33 and 34:
functional activities it could be s
Page 35 and 36:
Figure 1-6 Schematic showing the OR
Page 37 and 38:
RhlI Autoinducer RhlR Rhl Regulon G
Page 39 and 40:
1.5.2 Pseudomonas aeruginosa LES Th
Page 41 and 42:
2 Material and Methods 2 MATERIAL A
Page 43 and 44:
2.1.2 Strains used Pseudomonas aeru
Page 45 and 46:
Plasmids Plasmid Description Refere
Page 47 and 48:
In the case of capture vector const
Page 49 and 50:
Primer name Sequence Capture vector
Page 51 and 52:
The relevant homology sequence was
Page 53 and 54:
Important to the use of the capture
Page 55 and 56:
2.3.4 Electroporation: Transfer of
Page 57 and 58:
2.4.2 Infection dose preparation Th
Page 59 and 60:
mould was then added to the metal c
Page 61 and 62:
3 Analysis of genomic islands captu
Page 63 and 64:
3.1 Capture experiments involving g
Page 65 and 66:
DNA No of colonies Control No DNA 0
Page 67 and 68:
were designed based on the assumpti
Page 69 and 70:
3.2.1 KR115-lys10 and KR159-lys10 A
Page 71 and 72:
estriction digestion with I-SceI to
Page 73 and 74:
generated. The most significant nuc
Page 75 and 76:
they both produced three fragments
Page 77 and 78:
elated genomic islands and this res
Page 79 and 80:
All the Yersinia species available
Page 81 and 82:
Leicester). E105-leuX was the first
Page 83 and 84:
The strains highlighted with alignm
Page 85 and 86:
predict this protein to be a dnaJ-c
Page 87 and 88:
Figure 3-15 depicts the codon usage
Page 89 and 90:
ORF Gene range Blastx Description y
Page 91 and 92:
ecognition sequences are found with
Page 93 and 94:
(S: 150, E: 1e-37). This predicts t
Page 95 and 96:
having multiple copies of itself. A
Page 97 and 98:
The capture vectors used during thi
Page 99 and 100:
cloning and their findings was inco
Page 101 and 102:
The genomic island capture method c
Page 103 and 104: unpublished). This was achieved by
Page 105 and 106: Pseudomonas aeruginosa PA14 (UCBPP-
Page 107 and 108: stressful’ to the bacteria and th
Page 109 and 110: log CFU/mg 4 3 2 1 0 0 2 4 6 8 Days
Page 111 and 112: mice exhibited visible symptoms at
Page 113 and 114: 4.4.1 Survival times post-infection
Page 115 and 116: (P
Page 117 and 118: 4.4.4 Intravenous infection A quest
Page 119 and 120: Symptom score Symptom score Symptom
Page 121 and 122: Fold increase Fold increase 15 10 5
Page 123 and 124: Figure 4-15 Histopathology lung sec
Page 125 and 126: 4.4.7 Progression of disease post-i
Page 127 and 128: Figure 4-19 Leukocytes numbers moni
Page 129 and 130: interesting result is that the ∆P
Page 131 and 132: Unfortunately, 65% of the ORFs with
Page 133 and 134: morphology. ∆PAPI-1∆PAPI-2 leav
Page 135 and 136: The intravenous sepsis model data s
Page 137 and 138: in PAO1(Lee et al. 2006). They show
Page 139 and 140: shown that deletion of the exoU gen
Page 141 and 142: 5 The role of quorum-sensing in the
Page 143 and 144: Figure 5-1 shows overnight growth o
Page 145 and 146: Quorum-sensing is bacterial cell de
Page 147 and 148: Interestingly, the quorum-sensing d
Page 149 and 150: Symptom score Symptom score Symptom
Page 151 and 152: LES431 LESB58 LESB65 LES400 � �
Page 153: Additional comparisons of the CFU r
Page 157 and 158: For LESB58-infected mice the histop
Page 159 and 160: Strain Mouse Timepoint HB CI GC Sco
Page 161 and 162: Score: 4 HB Score: 4 Score: 4 B HCI
Page 163 and 164: post-infection to that of healthy m
Page 165 and 166: Tang et al. (1996) performed additi
Page 167 and 168: Figure 5-14 PFGE comparison of the
Page 169 and 170: isolates have a similar virulence i
Page 171 and 172: The data presented in this thesis s
Page 173 and 174: than PAO1. Despite the bacterial lu
Page 175 and 176: 6 Thesis conclusion The underlying
Page 177 and 178: Hopefully the methodologies describ
Page 179 and 180: 1 2 3 4 5 6 7 8 9 10 11 12 13 λHin
Page 181 and 182: AQ: 1 AQ: 2 AQ: 3 AQ: 4 AQ: 5 AQ: 6
Page 183 and 184: AQ: 18 AQ: 19 AQ: 20 F1 AQ: 21 tapr
Page 185 and 186: AQ: 32 AQ: 33 AQ: 34 tapraid5/z9d-j
Page 187 and 188: AQ: 40 AQ: 41 DISCUSSION tapraid5/z
Page 189 and 190: AQ: 48 tapraid5/z9d-jid/z9d-jid/z9d
Page 191 and 192: tapraid5/z9d-jid/z9d-jid/z9d01009/z
Page 193 and 194: 8 Bibliography AL-ALOUL, M., CRAWLE
Page 195 and 196: D'ARGENIO, D.A., WU, M., HOFFMAN, L
Page 197 and 198: genomic islands in Pseudomonas aeru
Page 199 and 200: MURRAY, A.W. and SZOSTAK, J.W., 198
Page 201 and 202: specificity of chaperone-usher mach
Page 203 and 204: VAN HEECKEREN, A.M. and SCHLUCHTER,
show all

5 The role of quorum-sensing in the virulence of Pseudomonas ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?