PHYSICS
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••,,<br />
""<br />
. .<br />
~: . , .. - .<br />
......,<br />
40<br />
}!<br />
'"......•;<br />
,<br />
- .-<br />
..-';": .<br />
',~<br />
t 'j ::<br />
;:. - . <<br />
HII<br />
a<br />
Fig. 21.14. Diffraction pattern<br />
obtained from a helical structure<br />
of DNA<br />
cross-wise pattern of the DNA X-ray<br />
diffraction picture (fig. 21.14) was<br />
due to a repeating helical structure.<br />
21.3.9. Polarization<br />
JIll<br />
b<br />
~, ",<br />
'.~<br />
Ull<br />
-- ",<br />
'~<br />
Ell<br />
,......,<br />
..<br />
, ..<br />
Fig. 21.15. Representation of the vector jj; of: a unpolarized light;<br />
b linearly polarized light; c circularly polarized light;<br />
d elliptically polarized light. The light ray is travelling at right<br />
angles to the plane of the paper<br />
152<br />
Light is electromagnetic radiation consisting of electric (jj;)<br />
and magnetic (H) vectors vibrating at right angles to each other<br />
and to the direction of propagation..Conventional polarization is<br />
associated with the electric vector. An ordinary beam of light is<br />
produced by a large number of waves emitted by the atoms or<br />
molecules of the light source; the direction of vibration of the jj;<br />
c<br />
\<br />
"',<br />
d<br />
, , ....<br />
,<br />
\, , ,<br />
I I<br />
I,<br />
vector changes about every 10- 8 seconds. A wave of unpolarized<br />
light traveling at right angles to a plane perpendicular to the<br />
direction of propagation is depicted in fig. 21.15, a.<br />
A wave is said to be linearly polarized if jj; vibrates in the same<br />
direction at all times at a particular point (fig. 21.15, b).<br />
If the tip of the vector jj; rotates in a circle with time, the<br />
wave is said to be circularly polarized (fig. 21.15, c).<br />
If the ti p of the vector jj; moves in an elli pse, the wave is<br />
said to be elliptically polarized (fig. 21.15, d).<br />
There are some special dichroic crystals (e.g., tourmaline)<br />
and Polaroid films (polyvinyl alcohol) that will pass light vibrating<br />
in only one direction. Such a crystal is called the polarizer.<br />
If the light is plane polarized after passing through the crystal, it<br />
will be stopped by an analyzer placed at right angles to the polarizer.<br />
Optical activity is the ability of certain materials to rotate the<br />
plane of polarization of transmitted light. The angle (cp) through<br />
which the light is rotated by the material depends on the length<br />
(l) of the sample and on the concentration (C) if the substance<br />
is in solution:<br />
Source<br />
of light<br />
II<br />
Polarizer<br />
21.3.10. Optical Activity<br />
qJ = aCI<br />
where a is the specific optical activity of the substance.<br />
a<br />
lj I<br />
- -1 1/7<br />
Optical cell<br />
Fig. 21.16. Polarymeter<br />
Analyizer<br />
(21.13)<br />
----+1 Registered<br />
system<br />
153